细胞因子预测鼻咽癌患者放疗敏感性及预后的相关性研究
发布时间：2019-05-05 07:54
【摘要】：目的：探讨鼻咽癌患者放疗前后细胞因子的表达与放疗敏感性及预后的关系。 方法：采用流式细胞小球微阵列术(Cytometric Bead Array, CBA)检测80例鼻咽癌患者放疗前后外周血血清中细胞因子IFN-γ、TNF-α、IL-2、IL-4、IL-5、IL-10的表达,并结合临床资料进行比较分析。 结果：IFN-γ和TNF-α的表达与鼻咽癌分期呈正相关,分期越早,表达越低(P值分别为0.05和0.02)。治疗前非角化型癌患者IFN-γ表达高于角化型癌患者(18.19vs.10.11,P=0.012)。放疗后IL-5和IL-10的表达较放疗前升高(4.25vs.3.50,P=0.000；3.02vs.2.58,P=0.020)。放疗前IL-5高表达的患者比低表达的患者疗效好,OR为0.19(95%CI：0.05_0.78,p=0.021),但与总生存无明显相关性。TNF-α和IL-2表达与患者预后相关,放疗前TNF-α高表达的患者比低表达的患者增加了超过两倍的死亡风险(HR=2.66,95%CI：1.04-6.78,p=0.041),相反,IL-2高表达的患者比低表达的患者有较低的死亡风险(HR=0.31,95%CI：0.13-0.75,p=0.009)。Kaplan-Meier曲线分析同样显示了放疗前IL-2和TNF-α的不同表达组曲线差异具有统计学意义。 结论：放疗前TNF-α的高表达、IL-2的低表达是鼻咽癌患者独立的不良预后因子。
[Abstract]:Objective: to investigate the relationship between the expression of cytokines and radiotherapy sensitivity and prognosis in patients with nasopharyngeal carcinoma (NPC) before and after radiotherapy. Methods: the expression of cytokines IFN- 纬, TNF- 伪 and IL-2,IL-4,IL-5,IL-10 in peripheral blood of 80 patients with nasopharyngeal carcinoma before and after radiotherapy was detected by flow cytometric microarray (Cytometric Bead Array, CBA). The clinical data were compared and analyzed. Results: the expression of IFN- 纬 and TNF- 伪 was positively correlated with the staging of NPC, and the earlier the staging, the lower the expression (P = 0 05 and 0 02, respectively). Before treatment, the expression of IFN- 纬 in patients with non-keratinous carcinoma was higher than that in patients with keratogenic carcinoma (18.19 vs.10.11, P < 0.012). The expression of IL-5 and IL-10 after radiotherapy was higher than that before radiotherapy (4.25 vs. 3.50, 0.000 卤3.52 vs 2.58, P = 0.020). The patients with high expression of IL-5 before radiotherapy had better curative effect than those with low expression, OR was 0.19 (95% CI = 0.05? 0.78, p = 0.021), but there was no significant correlation between the expression of TNF-伪 and IL-2 and the prognosis of the patients, but there was no significant correlation between the expression of TNF-伪 and the prognosis of the patients. Patients with high expression of TNF- 伪 before radiotherapy were more than twice as likely to die (HR=2.66,95%CI:1.04-6.78,p=0.041) as those with low expression. On the contrary, Patients with high expression of IL-2 have a lower risk of death (HR=0.31,95%CI:0.13-0.75,) than those with low expression. Kaplan-Meier curve analysis also showed that the curves of different expression groups of IL-2 and TNF- 伪 were significantly different before radiotherapy. Conclusion: the high expression of TNF- 伪 and the low expression of IL-2 before radiotherapy are independent adverse prognostic factors in NPC patients.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.63
本文编号：2469392
[Abstract]:Objective: to investigate the relationship between the expression of cytokines and radiotherapy sensitivity and prognosis in patients with nasopharyngeal carcinoma (NPC) before and after radiotherapy. Methods: the expression of cytokines IFN- 纬, TNF- 伪 and IL-2,IL-4,IL-5,IL-10 in peripheral blood of 80 patients with nasopharyngeal carcinoma before and after radiotherapy was detected by flow cytometric microarray (Cytometric Bead Array, CBA). The clinical data were compared and analyzed. Results: the expression of IFN- 纬 and TNF- 伪 was positively correlated with the staging of NPC, and the earlier the staging, the lower the expression (P = 0 05 and 0 02, respectively). Before treatment, the expression of IFN- 纬 in patients with non-keratinous carcinoma was higher than that in patients with keratogenic carcinoma (18.19 vs.10.11, P < 0.012). The expression of IL-5 and IL-10 after radiotherapy was higher than that before radiotherapy (4.25 vs. 3.50, 0.000 卤3.52 vs 2.58, P = 0.020). The patients with high expression of IL-5 before radiotherapy had better curative effect than those with low expression, OR was 0.19 (95% CI = 0.05? 0.78, p = 0.021), but there was no significant correlation between the expression of TNF-伪 and IL-2 and the prognosis of the patients, but there was no significant correlation between the expression of TNF-伪 and the prognosis of the patients. Patients with high expression of TNF- 伪 before radiotherapy were more than twice as likely to die (HR=2.66,95%CI:1.04-6.78,p=0.041) as those with low expression. On the contrary, Patients with high expression of IL-2 have a lower risk of death (HR=0.31,95%CI:0.13-0.75,) than those with low expression. Kaplan-Meier curve analysis also showed that the curves of different expression groups of IL-2 and TNF- 伪 were significantly different before radiotherapy. Conclusion: the high expression of TNF- 伪 and the low expression of IL-2 before radiotherapy are independent adverse prognostic factors in NPC patients.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.63
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