骨髓间充质干细胞治疗感音神经性耳聋相关实验技术体系建设概要

发布时间：2019-05-13 20:24

【摘要】：感音神经性耳聋是由于内耳感觉细胞的损伤造成的,其治疗一直是耳科医生十分棘手的问题。近年来,利用干细胞修复内耳感觉细胞在国内外大量的研究中取得了诸多重要进展,特别是骨髓间充质干细胞,有易分离、低免疫源性、无致瘤性等优点,极具临床应用价值。但目前国内外各个研究小组从干细胞的制备,诱导到移植内耳的方法均有差异,未形成统一的流程,不利于其学术交流和实验结果大样本收集。本文集中介绍谢鼎华课题组在国家973项目干细胞治疗耳聋的实验技术体系标准制定课题的基础研究方法,并综述目前本领域国际最新动态,初步提出骨髓间充质干细胞治疗感音神经性耳聋的技术方法和质量控制措施的推荐方案,为最终临床安全、规范化运用干细胞治疗耳聋提供依据。
[Abstract]:Sensorineural deafness is caused by the injury of sensory cells in the inner ear, and its treatment has always been a very difficult problem for otologists. In recent years, a lot of important progress has been made in the repair of inner ear sensory cells by stem cells at home and abroad, especially bone marrow mesenchymal stem cells, which have the advantages of easy separation, low immunogenicity and no tumorigenicity, so they have great clinical application value. However, at present, the methods of stem cell preparation, induction to inner ear transplantation are different in various research groups at home and abroad, and there is no unified process, which is not conducive to their academic exchange and large sample collection of experimental results. This paper focuses on the basic research methods of Xie Dinhua's experimental technical system standard for stem cell treatment of deafness in the national 973 project, and summarizes the latest international developments in this field. The technical methods and quality control measures of bone marrow mesenchymal stem cells in the treatment of sensorineural deafness were put forward, which provided the basis for the final clinical safety and standardized use of stem cells in the treatment of sensorineural deafness.
【作者单位】：中南大学湘雅二医院耳鼻咽喉头颈外科中南大学耳科研究所;解放军总医院耳鼻咽喉头颈外科解放军耳鼻咽喉研究所;
【基金】：国家重点基础研究发展计划(973):2012CB967900和2012CB967904 中南大学博士生自主创新项目:2016zzts139~~
【分类号】：R764.431

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