中性粒细胞型鼻息肉的临床特点及IL-17、IL-8的表达
发布时间：2019-05-27 11:46
【摘要】：目的:本文通过观察鼻息肉组织内的炎性细胞的浸润特点,分析以中性粒细胞的浸润为主的鼻息肉患者的临床特点,及IL-17、IL-8在其组织中的表达,从而为鼻息肉的临床分类和治疗策略提供依据。方法:收集我科行鼻内镜手术的患者鼻息肉组织,时间为2016年3月至2016年10月。根据病理特点,经HE染色观察后,采用Wenzel SE等[1]以炎性细胞的百分比例正常对照组的均数+2倍的标准差定义为该炎性细胞为主型的,区分出以中性粒细胞浸润为主的鼻息肉,并与嗜酸性粒细胞浸润为主的鼻息肉作比较,各收集40例,收集鼻中隔偏曲需要行矫正术的患者部分下鼻甲粘膜20例。常规检查鼻息肉患者的外周血中性粒细胞百分比、嗜酸性粒细胞百分比,通过鼻息肉患者的术前鼻窦CT、模拟视觉量表(VAS)及鼻内镜下观察的临床数据,分析其临床特点,并通过免疫组化检查IL-17和IL-8在组织中的。结果:本实验中性粒细胞4%(1.25%+2×1.37%=3.96)定义为中性粒细胞型鼻息肉;嗜酸性粒细胞大于8%(4.4%+2×1.7%=7.9%)定义为嗜酸性粒细胞型鼻息肉。中性粒细胞浸润为主的鼻息肉组患者与嗜酸性粒细胞型鼻息肉组患者相比较,在性别、年龄、中性粒细胞百分比无显著差别(p05.0);NEUNP与ENP患者流粘脓涕程度分别为6.35±1.20和5.69±1.31(p05.0),存在显著的差异;而两组患者的VAS整体主观症状、鼻内镜下鼻息肉病变程度均无显著差别(p05.0);NEUNP 患者和EOSNP患者的鼻窦CT影像学整体得分分别为9.59±3.35和14.43±3.81(p05.0),上颌窦评分分别为3.14±1.21和2.05±1.02(p05.0),均有显著的差异,即NEUNP患者的整体病变较轻,但上颌窦病变较严重。IL-17、IL-8在NEUNP中的表达均明显高于在EOSNP、正常下鼻甲粘膜中的表达(p05.0)。结论:中性粒细胞型鼻息肉患者的分泌物为粘脓性分泌物,其主观症状的评分明显高于嗜酸性粒细胞鼻型的鼻息肉患者;中性粒细型鼻息肉患者上颌窦的病变程度同样明显高于嗜酸性粒细胞鼻息肉患者,整体上鼻窦的病变程度相对嗜酸性粒细胞型鼻息肉较轻。IL-17、IL-8在中性粒细型鼻息肉组织中的表达均高于嗜酸性粒细胞型鼻息肉和正常对照组,提示IL-17、IL-8趋化中性粒细胞作用于鼻息肉,对中性粒细胞型鼻息肉的发病机制发挥重要作用。因此,以中性粒细胞浸润为主的鼻息肉,有明显的临床和病理特点,为鼻息肉的发病机制提供了新的认识。
[Abstract]:Objective: to observe the infiltration characteristics of inflammatory cells in nasal polyps, to analyze the clinical characteristics of patients with nasal polyps dominated by neutrophil infiltration, and to analyze the expression of IL-17,IL-8 in nasal polyps. So as to provide the basis for the clinical classification and treatment strategy of nasal polyps. Methods: the nasal polyp tissues of patients undergoing endoscopic sinus surgery were collected from March 2016 to October 2016. According to the pathological characteristics, after HE staining, Wenzel SE et al. [1] were defined as the main type of inflammatory cells by using Wenzel SE et al. [1] the standard deviation of the percentage of inflammatory cells in the normal control group was 2 times that of the normal control group. Nasal polyps with neutrophil infiltration were distinguished and compared with eosinophils infiltrating nasal polyposis. 40 cases were collected and 20 cases of inferior turbinate mucosa needed correction in patients with deviation of nasal septum. The percentage of neutrophils and eosinophils in peripheral blood of patients with nasal polyposis were examined by routine examination, and the clinical characteristics were analyzed by CT, simulated visual scale (VAS) and endoscopic observation in patients with nasal polyps. The expression of IL-17 and IL-8 in the tissue was examined by immunohistochemistry. Results: neutrophil 4% (1.25% 2 脳 1.37% = 3.96) was defined as neutrophil nasal polyp, and eosinophils more than 8% (4.4% 2 脳 1.7% = 7.9%) were defined as eosinophilic nasal polyp. There was no significant difference in sex, age and percentage of neutrophils between the patients with nasal polyposis with neutrophil infiltration and those with eosinophilic nasal polyposis (p05.0). The degree of runny nose in patients with NEUNP and ENP was 6.35 卤1.20 and 5.69 卤1.31 (p05.0), respectively, but there was no significant difference between the two groups in the overall subjective symptoms of VAS and the degree of nasal polyps under nasal endoscope (p05.0). The overall scores of CT and maxillary sinus in NEUNP patients and EOSNP patients were 9.59 卤3.35 and 14.43 卤3.81 (p05.0), respectively, and the maxillary sinus scores were 3.14 卤1.21 and 2.05 卤1.02 (p05.0), respectively. That is to say, the overall lesions of NEUNP patients were mild, but the maxillary sinus lesions were more serious. The expression of IL-17 and IL 鈮，
本文编号：2486122
[Abstract]:Objective: to observe the infiltration characteristics of inflammatory cells in nasal polyps, to analyze the clinical characteristics of patients with nasal polyps dominated by neutrophil infiltration, and to analyze the expression of IL-17,IL-8 in nasal polyps. So as to provide the basis for the clinical classification and treatment strategy of nasal polyps. Methods: the nasal polyp tissues of patients undergoing endoscopic sinus surgery were collected from March 2016 to October 2016. According to the pathological characteristics, after HE staining, Wenzel SE et al. [1] were defined as the main type of inflammatory cells by using Wenzel SE et al. [1] the standard deviation of the percentage of inflammatory cells in the normal control group was 2 times that of the normal control group. Nasal polyps with neutrophil infiltration were distinguished and compared with eosinophils infiltrating nasal polyposis. 40 cases were collected and 20 cases of inferior turbinate mucosa needed correction in patients with deviation of nasal septum. The percentage of neutrophils and eosinophils in peripheral blood of patients with nasal polyposis were examined by routine examination, and the clinical characteristics were analyzed by CT, simulated visual scale (VAS) and endoscopic observation in patients with nasal polyps. The expression of IL-17 and IL-8 in the tissue was examined by immunohistochemistry. Results: neutrophil 4% (1.25% 2 脳 1.37% = 3.96) was defined as neutrophil nasal polyp, and eosinophils more than 8% (4.4% 2 脳 1.7% = 7.9%) were defined as eosinophilic nasal polyp. There was no significant difference in sex, age and percentage of neutrophils between the patients with nasal polyposis with neutrophil infiltration and those with eosinophilic nasal polyposis (p05.0). The degree of runny nose in patients with NEUNP and ENP was 6.35 卤1.20 and 5.69 卤1.31 (p05.0), respectively, but there was no significant difference between the two groups in the overall subjective symptoms of VAS and the degree of nasal polyps under nasal endoscope (p05.0). The overall scores of CT and maxillary sinus in NEUNP patients and EOSNP patients were 9.59 卤3.35 and 14.43 卤3.81 (p05.0), respectively, and the maxillary sinus scores were 3.14 卤1.21 and 2.05 卤1.02 (p05.0), respectively. That is to say, the overall lesions of NEUNP patients were mild, but the maxillary sinus lesions were more serious. The expression of IL-17 and IL 鈮，
本文编号：2486122
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