人工耳蜗植入术后感染（细菌生物膜）的基础和临床研究

发布时间：2019-05-27 14:15

【摘要】：目的：构建BBF在耳蜗植入术后感染动物体内模型，及形态学观察；观察表皮葡萄球菌的体内外BBF的形成、结构形态模型；探讨临床上清除及抑制细菌生物膜形成的相关治疗措施。方法：建立表皮葡萄球菌生物膜的体外模型，利用扫描电镜观察新西兰兔CI术后体内BBF感染模型。结果：①成功建立了表皮葡萄球菌的体外生物膜，用刚果红培养基对BBF检测进行初筛；②成功构建耳蜗植入术后感染新西兰兔体内模型；③扫描电镜下成功观察到典型的BBF形态结构，，细菌间纤维样物质相互粘连，细菌形态，球形的表皮葡萄球菌成团分布、聚集，呈多层细胞结构，纤维样粘多糖和菌体间的连结，菌丝、细胞外基质（ECM）相互交错，形成致密的膜网状空间结构；④临床上，经过综合处理，采用2步骤的方法，使用化学制剂如H2O2、浓碘伏可有效清除和抑制细菌生物膜的形成。结论：①本实验研究结果观察到了表皮葡萄球菌生物膜体外模型的结构，对其表型进行初步检测，了解其生长动力学，证明了表皮葡萄球菌生物膜具有强大的致病性；②新西兰兔体内生物膜的形态得到证实，通过扫描电镜下可以直观的观察到人工耳蜗植入术后感染新西兰兔植入体周围S.E.生物膜的结构，为进一步研究人工耳蜗植入术后感染提供了实验的模型和支持；③临床上利用细菌生物膜清除剂有3%过氧化氢溶液、碘伏等，可有效清除和抑制细菌生物膜的形成。
[Abstract]:Objective: to construct the in vivo model and morphological observation of BBF in infected animals after cochlear implantation, and to observe the formation and structural morphology model of BBF in vitro and in vivo of Staphylococcus epidermidis. To explore the clinical treatment measures to clear and inhibit the formation of bacterial biofilm. Methods: the in vitro model of Staphylococcus epidermidis biofilm was established. The model of BBF infection in New Zealand rabbits after CI was observed by scanning electron microscope (SEM). Results: (1) the biofilm of Staphylococcus epidermidis in vitro was successfully established and screened for BBF with Congo red culture medium, and (2) the model of infected New Zealand rabbits after cochlear implantation was successfully constructed. (3) the typical morphology and structure of BBF were successfully observed under scanning electron microscope (SEM). The fiber-like substances adhered to each other, the bacterial morphology, the cluster distribution and aggregation of spherical Staphylococcus epidermidis, and the connection between fibroid mucopolysaccharide and bacteria. The hyphae and extracellular matrix (ECM) interlaced with each other to form a dense membrane reticular spatial structure. (4) after comprehensive treatment and two-step method, concentrated iodine volt can effectively eliminate and inhibit the formation of bacterial biofilm by using chemical preparations such as H _ 2O _ 2. Conclusion: 1 the structure of Staphylococcus epidermidis biofilm model in vitro was observed, the phenotype of Staphylococcus epidermidis biofilm was detected, the growth kinetics of Staphylococcus epidermidis biofilm was understood, and it was proved that Staphylococcus epidermidis biofilm had strong pathogenicity. 2 the morphology of biofilm in New Zealand rabbits was confirmed. The infection around the implantation of New Zealand rabbits after cochlear implantation could be observed intuitively by scanning electron microscope. The structure of biofilm provides experimental model and support for further study of infection after cochlear implantation. (3) there are 3% hydrogen peroxide solution, iodophor and so on, which can effectively eliminate and inhibit the formation of bacterial biofilm by using bacterial biofilm remover.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R764.93

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