喉鳞状细胞癌血清中抗survivin抗体和survivin mRNA的相关性和潜在生物标志物作用的研究

发布时间：2019-07-08 21:27

【摘要】：目的： 1、了解喉鳞状细胞癌（简称喉鳞癌）患者外周血清中抗survivin抗体和survivin mRNA的相关性。 2、比较抗survivin抗体、抗p53抗体和抗c-myc抗体作为生物标志物在喉鳞癌检出中的作用，分析联合检测的价值。 3、探讨血清生物标志物在喉鳞癌早期诊断及发生、发展和预后监测方面的临床意义。方法： 1、酶联免疫吸附试验(ELISA)用于检测血清样本中抗survivin抗体、抗p53抗体和抗c-myc抗体的表达。 2、半定量聚合酶链式反应（RT-PCR）检测相同样本中survivinmRNA的表达。结果： 1、抗survivin抗体和survivin mRNA在喉鳞癌患者外周血清中高表达，，特别是有颈淋巴结转移的样本，且二者的表达水平显著相关。 2、抗p53抗体和抗c-myc抗体在相同样本中同样高表达。 3、在接受根治性手术治疗后抗survivin抗体与survivin mRNA的表达水平显著下降，抗p53抗体和抗c-myc抗体得到了类似的结果。 4、抗survivin抗体、抗p53抗体和抗c-myc抗体单独检测时均获得了较高的阳性率，三种抗体联合检测的阳性率并不优于抗survivin抗体的阳性率，但优于抗p53抗体和抗c-myc抗体。结论： 1、喉鳞癌外周血清中抗survivin抗体与survivin mRNA的表达水平相关。 2、抗survivin抗体可能是喉鳞癌的一种有价值的生物标志物。Survivin在喉鳞癌的发生、发展进程中可能扮演重要角色。 3、联合检测三种不同的肿瘤标志物并不能有效提高喉鳞癌的术前检出率，但可能在监测手术效果方面具有积极意义。
文内图片：

图片说明：A运用ELISA夹心法测定喉鳞癌患者（LSCC，n=79）和正常人血清（NHS，n=47）
[Abstract]:Objective: 1. To investigate the correlation between anti-survivin antibody and survivin mRNA in peripheral serum of patients with laryngeal squamous cell carcinoma (LSC). 2. To compare the role of anti-survivin antibody, anti-p53 antibody and anti-c-myc antibody as biomarkers in the detection of laryngeal squamous cell carcinoma, and to analyze the value of combined detection. 3. To investigate the clinical significance of serum biomarkers in early diagnosis, occurrence, development and prognosis monitoring of laryngeal squamous cell carcinoma. Methods: 1. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of anti-survivin antibody, anti-p53 antibody and anti-c-myc antibody in serum samples. 2. Semi-quantitative polymerase chain reaction (RT-PCR) was used to detect the expression of survivinmRNA in the same sample. Results: 1. Anti-survivin antibody and survivin mRNA were highly expressed in peripheral serum of patients with laryngeal squamous cell carcinoma, especially in samples with cervical lymph node metastasis, and their expression levels were significantly correlated. 2. Anti-p53 antibody and anti-c-myc antibody were also highly expressed in the same sample. 3. The expression of anti-survivin antibody and survivin mRNA decreased significantly after radical operation, and similar results were obtained between anti-p53 antibody and anti-c-myc antibody. 4. The positive rates of anti-survivin antibody, anti-p53 antibody and anti-c-myc antibody were high when detected alone. The positive rate of the three antibodies was not better than that of anti-survivin antibody, but better than that of anti-p53 antibody and anti-c-myc antibody. Conclusion: 1. The expression of anti-survivin antibody in peripheral serum of laryngeal squamous cell carcinoma is correlated with the expression of survivin mRNA. 2. Anti-survivin antibody may be a valuable biomarker of laryngeal squamous cell carcinoma. Survivin may play an important role in the occurrence and development of laryngeal squamous cell carcinoma. 3. The combined detection of three different tumor markers can not effectively improve the preoperative detection rate of laryngeal squamous cell carcinoma, but it may have positive significance in monitoring the surgical effect.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.65

【参考文献】