乙肝病毒血清标志物与HBV DNA载量及肝功能指标的关联性

发布时间：2018-03-16 01:15

本文选题：乙型肝炎病毒　切入点：乙肝病毒血清标志物　出处：《华中科技大学学报(医学版)》2017年02期 　论文类型：期刊论文

【摘要】：目的探讨外周血中乙肝病毒血清标志物(hepatitis B virus serum markers,HBV-M)、HBV DNA载量和反映肝损伤的肝功能指标之间的关联性。方法回顾分析2014年3月~2016年3月同济医院483例慢性乙型肝炎患者资料,HBV-M采用微粒子化学发光技术定量检测;HBV DNA采用实时荧光定量PCR检测;谷丙转氨酶(ALT)和谷草转氨酶(AST)采用紫外连续监测法检测。结果 HBsAg的含量与HBV DNA载量、ALT异常比例(41U/L)和AST异常比例(35U/L)之间均无关联性(均P0.05);HBeAg的含量与HBV DNA载量和ALT异常比例之间均无关联性(均P0.05),但与AST异常比例有关联,关联程度不密切(r=0.21,P0.01);抗-HBe的含量与HBV DNA载量和AST异常比例之间有关联性,但关联程度不密切(r=0.16,P0.05;r=0.19,P0.01),与ALT异常比例之间无关联性(P0.05);抗-HBc的含量与HBV DNA载量、ALT异常比例和AST异常比例之间均有关联性,但关联程度不密切(r=0.25,P0.01;r=0.29,P0.01;r=0.29,P0.01);HBV DNA载量的对数值与ALT和AST呈正相关,但线性相关程度较弱(r=0.24;r=0.29)。结论乙肝病毒血清标志物定量检测和HBV DNA定量检测,二者相互补充但不能取代,同时联合肝功能各项指标的检测,更有助于了解肝组织的受损程度。
[Abstract]:Objective to investigate the relationship between hepatitis B virus serum markers HBV DNA load and liver function index reflecting liver injury in peripheral blood. Methods from March 2014 to March 2016, #number0# cases of chronic hepatitis B in Tongji Hospital were retrospectively analyzed. HBV-M was quantitatively detected by microparticle chemiluminescence technique and real-time fluorescence quantitative PCR was used to detect HBV DNA. Alt and AST) were detected by UV continuous monitoring. Results there was no correlation between the HBsAg content and the abnormal ratio of alt in HBV DNA and AST at 35 U / L (both P0.05HBeAg and HBV DNA load and ALT). There was no correlation between abnormal proportions (P0.05%, P 0.05), but there was no correlation between abnormal proportions of AST, There was a correlation between anti-HBe content and HBV DNA load and AST abnormal ratio. However, the correlation degree was not close, but there was no correlation between the ratio of ALT abnormality and the level of anti-HBc, but there was a positive correlation between the content of anti-HBc and the abnormal ratio of HBV DNA load and alt and AST, but the correlation degree was not close, but the logarithmic value of DNA load of ALT was positively correlated with ALT and AST. Conclusion the quantitative detection of serum markers of hepatitis B virus and the quantitative detection of HBV DNA complement each other, but can not be replaced, and combined with the detection of various indexes of liver function, it is more helpful to understand the degree of damage to liver tissue.
【作者单位】：华中科技大学同济医学院附属同济医院检验科;华中科技大学同济医学院基础医学院病原生物学系;
【分类号】：R446.6;R512.62

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