HBV相关慢加急肝衰竭患者血浆抗可溶性肝抗原抗体的检测及意义

发布时间：2018-04-30 15:26

本文选题：抗可溶性肝抗原抗体 + HBV相关慢加急肝衰竭　；参考：《广东医学》2017年13期

【摘要】：目的评价血浆抗可溶性肝抗原抗体(抗SLA)检测在乙型肝炎病毒(HBV)相关慢加急肝衰竭(HA)患者中的意义。方法使用抗SLA酶联免疫检测试剂盒,检测H-A患者(H-A组)、肝硬化(LC)患者(LC组)、慢性乙型肝炎(CHB)患者(CHB组)和健康对照者(HC组)血浆抗SLA,分析不同临床组间抗SLA的表达差异,以及与肝功能相关指标的关系。结果慢性HBV感染(CHB+LC+H-A)组的抗SLA水平均显著高于HC组(P0.001),而H-A组抗SLA的OD值显著高于CHB组(P0.001)、LC组(P0.001);在HBV感染者中,抗SLA的OD值水平与ALT(r=0.260,P=0.046)及TBIL(r=0.646,P0.001)水平呈正相关,与HBV-DNA无明显相关性(r=-0.069,P=0.598)。在H-A组,抗SLA的OD值水平与总胆汁酸呈正相关(r=0.478,P=0.024),与凝血酶原活动度、丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、直接胆红素(DBIL)、总胆红素(TBIL)无显著相关性。结论这种检测方法可用于评估不同慢性HBV感染者抗SLA的表达差异,H-A患者高表达抗SLA可能与肝细胞的严重损伤及免疫发病机制相关。
[Abstract]:Objective to evaluate the significance of plasma anti soluble liver antigen antibody (anti SLAs) in patients with hepatitis B virus (HBV) associated chronic acute hepatic failure (HAF). Methods the plasma anti-SLAs of H-A patients, liver cirrhosis patients (LC group), chronic hepatitis B group (SLA group) and healthy control group (HC group) were detected by using anti SLA enzyme linked immunosorbent assay (Elisa) kit. The difference of anti SLA expression among different clinical groups was analyzed. And the relationship between liver function and related indexes. Results the level of anti SLA in chronic HBV infection group was significantly higher than that in HC group, while the OD value of anti SLA in H-A group was significantly higher than that in CHB group P0.001 / LC group (P 0.001). In HBV infected patients, the OD level of anti SLA was positively correlated with the levels of Altrin 0.260P0.046) and TBILrrnr 0.646P0.001), but had no significant correlation with HBV-DNA. In H-A group, the OD level of anti SLA was positively correlated with total bile acid, but not with prothrombin activity, alanine aminotransferase, aspartate aminotransferase, direct bilirubin and total bilirubin. Conclusion this method can be used to evaluate the expression difference of anti SLA in patients with chronic HBV infection. The high expression of anti SLA in patients with H-A may be related to the severe injury of hepatocytes and the mechanism of immune pathogenesis.
【作者单位】：南方医科大学南方医院感染内科;广州市第八人民医院肝病科;
【基金】：国家自然科学基金资助项目(编号:81270025)
【分类号】：R512.62;R575.3

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