骨髓间充质干细胞尾静脉注射治疗大鼠肝损伤的效果及机制

发布时间：2018-05-16 04:12

本文选题：骨髓间充质干细胞 + 肝损伤　；参考：《山东医药》2017年03期

【摘要】：目的观察骨髓间充质干细胞尾静脉注射治疗大鼠肝损伤的效果,并探讨其机制。方法将30只SD大鼠随机分为对照组、模型组和治疗组,每组10只。模型组和治疗组大鼠用连续4个月皮下注射D-半乳糖的方法成功制备大鼠肝损伤模型。治疗组大鼠造模成功后尾静脉注射3×106个用绿色荧光蛋白标记的间充质干细胞,对照组注射同等剂量的生理盐水。3 d后检测各组大鼠血清天冬氨酸转氨酶(AST)、谷氨酸转氨酶(ALT)。处死各组大鼠,取肝组织制备冰冻切片,荧光显微镜下观察间充质干细胞分布,行HE染色观察肝组织病理结构;取肝组织制成匀浆,用黄嘌呤氧化酶法检测超氧化物歧化酶(SOD),用硫代巴比妥酸法检测丙二醛(MDA),采用实时定量PCR法检测肝组织中的p53、p21 mRNA。结果治疗组大鼠注射骨髓间充质干细胞后3 d,荧光显微镜下可见带绿色荧光的骨髓间充质干细胞散在于肝脏内。光镜下观察,对照组大鼠肝细胞形态及结构正常,肝小叶结构正常;模型组大鼠肝组织水肿,细胞边缘不完整,肝索排列紊乱,肝小叶结构不清;与模型组比较,治疗组大鼠肝细胞形态较规则,肝小叶结构清晰,病理损伤减轻。与对照组相比,模型组大鼠肝组织中SOD水平降低,MDA水平升高,p53、p21 mRNA表达上调,血清ALT、AST水平升高(P均0.05)。与模型组相比,治疗组大鼠肝组织中SOD水平升高,MDA水平降低,p53、p21 mRNA表达下调,血清ALT、AST水平降低(P均0.05)。结论骨髓间充质干细胞静注能起到治疗D-半乳糖诱导的大鼠肝损伤的效果,其机制可能与下调大鼠肝组织中p53、p21 mRNA表达及减轻肝组织氧化应激水平有关。
[Abstract]:Objective to observe the effect of tail vein injection of bone marrow mesenchymal stem cells on liver injury in rats. Methods 30 SD rats were randomly divided into control group, model group and treatment group with 10 rats in each group. Rats in the model group and the treatment group were successfully induced liver injury by subcutaneous injection of D-galactose for 4 months. In the treatment group, 3 脳 106 mesenchymal stem cells labeled with green fluorescent protein were injected into the tail vein after successful modeling. The serum aspartate aminotransferase (AST) and glutamate aminotransferase (alt) were detected in the control group after 3 days of injection with the same dose of normal saline. Rats in each group were killed, liver tissue was taken to prepare frozen sections, mesenchymal stem cells were observed under fluorescence microscope, pathological structure of liver tissue was observed by HE staining, liver tissue was made into homogenate, Superoxide dismutase (SOD) was detected by xanthine oxidase method, malondialdehyde (MDAA) was detected by thiobarbituric acid method, and p53 ~ + p21 mRNA in liver tissue was detected by real-time quantitative PCR. Results 3 days after injection of bone marrow mesenchymal stem cells in the treatment group, the green fluorescent mesenchymal stem cells were found in the liver under fluorescence microscope. Under light microscope, the morphology and structure of hepatocytes and lobules were normal in control group, edema of liver tissue, incomplete cell edge, disordered arrangement of hepatic cord and unclear structure of hepatic lobule in model group. In the treatment group, the morphology of liver cells was regular, the structure of hepatic lobules was clear, and the pathological injury was alleviated. Compared with the control group, the level of SOD in the liver tissue of the model group decreased and the expression of p53 + p21 mRNA was up-regulated, and the level of serum alt was increased (P < 0.05). Compared with the model group, the level of SOD in the liver tissue of the treatment group was increased and the expression of p53, p21 mRNA was down-regulated, while the level of serum alt was decreased in the treatment group (P < 0.05). Conclusion intravenous injection of bone marrow mesenchymal stem cells can treat the liver injury induced by D-galactose in rats. The mechanism may be related to the down-regulation of p53 mRNA expression and the reduction of oxidative stress in liver tissue.
【作者单位】：福建省立医院;
【分类号】：R551.3

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