短暂禁食减轻四氯化碳诱导的小鼠急性肝炎

发布时间：2018-05-16 04:22

本文选题：短暂禁食 + 四氯化碳　；参考：《基因组学与应用生物学》2017年02期

【摘要】：短暂禁食可引起包括糖皮质激素释放在内的一系列神经内分泌改变,对于疾病防治具有独特而重要的意义。本研究观察了短暂禁食对四氯化碳(carbon tetrachloride,CCl_4)诱导的急性肝炎小鼠肝组织损伤程度及炎症反应水平的影响及其与糖皮质激素之间的可能关联。结果发现,短暂禁食可明显下调CCl_4处理小鼠血浆中天冬氨酸转氨酶(aspartate transaminase,AST)及丙氨酸转氨酶(alanine transaminase,ALT)水平,显著减轻肝组织病理学改变,对肝组织内还原型谷胱甘肽(glutathione,GSH)含量无明显影响但可显著降低血浆中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)及白介素-6(interleukin-6,IL-6)浓度。糖皮质激素受体拮抗剂米非司酮不能阻断短暂禁食对转氨酶水平的下调作用,糖皮质激素受体激活剂地塞米松也不能模拟短暂禁食对转氨酶水平的下调作用。以上结果提示:短暂禁食可通过不依赖于糖皮质激素的途径抑制炎症反应、从而减轻CCl_4诱导的急性肝炎。
[Abstract]:Temporary fasting can cause a series of neuroendocrine changes including glucocorticoid release, which has a unique and important significance for the prevention and treatment of diseases. In this study, the effects of transient fasting on the degree of liver injury and inflammatory response in mice with acute hepatitis induced by carbon tetrachloride (CCL _ 4) and its possible association with glucocorticoid were observed. The results showed that the levels of aspartate transaminase (aspartate transaminase) and alanine transaminase (alt) in plasma of CCl_4 treated mice were significantly down-regulated by transient fasting, and the histopathological changes of liver were significantly alleviated. The content of glutathione (GSH) in liver tissue was not significantly affected, but the concentrations of tumor necrosis factor- 伪 (TNF- 伪) and interleukin-6 (IL-6) in plasma were significantly decreased. Mifepristone, a glucocorticoid receptor antagonist, could not block the down-regulation of transaminase level by transient fasting, nor could dexamethasone, a glucocorticoid receptor activator, mimic the down-regulation of transaminase level by transient fasting. These results suggest that transient fasting can inhibit inflammatory response through a glucocorticoid independent pathway, thereby alleviating acute hepatitis induced by CCl_4.
【作者单位】：重庆医科大学病理生理学教研室;重庆文理学院校医院;重庆医科大学干细胞与组织工程研究室;
【基金】：国家自然科学基金项目(No.81370179;No.81671953) 重庆市科委自然科学基金项目(cstc2016jcyj A0039)共同资助
【分类号】：R575.1

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