急性肝衰竭大鼠肝脏的蛋白质组学研究

发布时间：2018-05-21 04:11

  本文选题：大鼠 + 肝功能衰竭　； 参考：《重庆医学》2015年12期

【摘要】：目的筛选急性药物性肝衰竭大鼠肝脏组织的差异表达蛋白质。方法将24只SD大鼠分为两组,12只通过腹腔注射10g/L的D-氨基半乳糖建立大鼠急性肝衰竭模型(实验组),12只腹腔注射生理盐水作为对照组。提取两组大鼠肝脏组织的蛋白质,定量后进行IEF和十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)双向凝胶电泳(2-DE)分离,通过软件找到差异蛋白点并使用基质辅助激光解吸电离飞行时间质谱法(MALDI-TOF-MS)进行鉴定。结果成功鉴定出27个有效的差异蛋白质点,其中实验组较对照组上调15个,下调12个。结论急性药物性肝衰竭模型大鼠肝脏酪蛋白激酶Iα(CKⅠα)、酪氨酸蛋白激酶(PTK)、增殖细胞核抗原(PCNA)等蛋白的表达较正常大鼠存在显著差异。
[Abstract]:Objective to screen differentially expressed proteins in the liver tissues of rats with acute drug-induced liver failure. Methods Twenty-four Sprague-Dawley rats were divided into two groups: 12 rats were treated by intraperitoneal injection of D-galactosamine (10g/L) to establish acute hepatic failure model (experimental group: 12 rats were treated by intraperitoneal injection of normal saline as control group). Two groups of rat liver tissue proteins were extracted and separated by IEF and SDS-PAGE2-DEG after quantitative analysis. Differential protein sites were found by software and identified by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). Results 27 effective differentially expressed protein spots were successfully identified, of which 15 were up-regulated and 12 down-regulated in the experimental group as compared with the control group. Conclusion the expressions of tyrosine kinase I 伪 -CK 鈪，

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