过氧化氢酶抑制剂氨基三唑减轻急性酒精性肝损伤

发布时间：2018-07-07 23:23

本文选题：过氧化氢酶 + 酒精性肝损伤　；参考：《生理学报》2015年01期

【摘要】：本文旨在通过观察过氧化氢酶(catalase,CAT)抑制剂氨基三唑(aminotriazole,ATZ)对酒精诱导的急性肝损伤的影响,初步探讨CAT在酒精性肝损伤中的可能作用。以雄性Sprague Dawley(SD)大鼠为实验对象,采用酒精腹腔注射诱导急性肝损伤,在造模前30 min腹腔注射不同剂量ATZ(100~400 mg/kg)或相同体积溶剂(对照)。造模24 h后,检测大鼠血浆天冬氨酸转氨酶(aspartate transaminase,AST)、丙氨酸转氨酶(alanine transaminase,ALT)及乳酸脱氢酶(lactate dehydrogenase,LDH)水平,用HE染色法观察肝组织病理改变程度,用试剂盒检测肝组织内CAT活性、过氧化氢(hydrogen peroxide,H2O2)水平及丙二醛(malondialdehyde,MDA)含量,ELISA法检测血浆中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)及白介素-6(interleukin-6,IL-6)水平。结果显示,ATZ处理可剂量依赖性降低酒精暴露大鼠血浆中ALT、AST及LDH水平,并减轻酒精诱导的肝组织病理损伤;ATZ可抑制酒精暴露大鼠肝组织内CAT活性、降低H2O2水平及MDA含量;ATZ也可下调酒精暴露大鼠血浆中TNF-α和IL-6水平。以上结果表明,ATZ可减轻酒精诱导的大鼠急性肝损伤,提示CAT可能在酒精性肝损伤中发挥了重要的致病作用。
[Abstract]:The aim of this study was to investigate the possible role of cat in alcoholic liver injury by observing the effect of aminotriazole (ATZ), an inhibitor of catalase (cat), on acute hepatic injury induced by alcohol. Acute liver injury was induced by intraperitoneal injection of alcohol in male Sprague Dawley (SD) rats. Different doses of ATZ (100,400 mg/kg) or the same volume solvent (control group) were injected intraperitoneally 30 min before modeling. The plasma levels of aspartate transaminase (aspartate), alanine transaminase (alt) and lactate dehydrogenase (lactate) were measured after 24 hours of modeling. The pathological changes of liver tissue were observed by HE staining, and the activity of cat in liver tissue was detected by kit. The levels of hydrogen peroxide-H _ 2O _ 2 and malondialdehyde (malondialdehyde) were determined by Elisa. The levels of tumor necrosis factor 伪 (tumor necrosis factor- 伪 (TNF- 伪) and interleukin-6 (IL-6) in plasma were determined by Elisa. The results showed that ATZ treatment could decrease the levels of alt AST and LDH in plasma in a dose-dependent manner, and attenuate the pathological injury of liver tissue induced by alcohol. ATZ could inhibit the cat activity in the liver tissue of alcohol exposed rats. The decrease of H _ 2O _ 2 and MDA content and ATZ could also down-regulate the levels of TNF- 伪 and IL-6 in plasma of rats exposed to alcohol. These results suggest that ATZ can attenuate acute hepatic injury induced by alcohol in rats, suggesting that cat may play an important role in alcoholic liver injury.
【作者单位】：重庆医科大学生理学教研室;重庆医科大学病理生理学教研室;重庆文理学院校医院;
【基金】：supported by the National Natural Science Foundation of China(No.81370179)
【分类号】：R575

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