趋化因子2促进肝再生中脂肪的形成

发布时间：2018-10-21 19:18

【摘要】：目的探讨趋化因子2(CCL2)对肝再生的影响以及作用机制。方法大鼠随机分为3组,每组10只。液压转基因技术将质粒转入大鼠体内,6 h后荧光显微镜下观察转染效率。称量再生肝重量,计算肝再生率和肝脏指数以观察肝脏再生情况。测量血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)与总胆红素(TBIL)的含量以评估肝脏功能情况。苏丹Ⅳ染色观测脂肪聚积情况。Real-time PCR检测脂肪代谢相关基因的表达。Western blotting检测磷酸化丝裂原细胞外激酶1/2(p-MEK1/2)和磷酸化细胞外信号调节激酶l/2(p-ERKl/2)的表达情况。结果转质粒后6 h各组绿色荧光蛋白表达量均大于30%。p EGFP-N1-CCL2转染组肝再生率、肝脏指数、ALT、AST和TBIL含量均高于p EGFP-N1组。随转基因时间延长,脂肪代谢相关基因表达量增加,有较多猩红色脂肪滴出现,pMEK1/2和p-ERKl/2表达量增多。结论趋化因子2可能通过MEK/ERK通路增加脂肪合成,促进肝脏再生。
[Abstract]:Objective to investigate the effect and mechanism of chemokine 2 (CCL2) on liver regeneration. Methods the rats were randomly divided into 3 groups with 10 rats in each group. The transfection efficiency was observed under fluorescence microscope for 6 hours. Weight of regenerated liver, liver regeneration rate and liver index were calculated to observe liver regeneration. Serum alanine aminotransferase (ALT), glutamic oxalacetic transaminase (AST) and total bilirubin (TBIL) were measured to evaluate liver function. Real-time PCR was used to detect the expression of genes related to fat metabolism. The expression of extracellular phosphomitogen kinase 1 / 2 (p-MEK1/2) and phosphorylated signal regulated kinase l / 2 (p-ERKl/2) were detected by Real-time PCR. Results the expression of green fluorescent protein in each group was higher than that in 30 路p EGFP-N1-CCL2 group at 6 h after transfection, and the liver regeneration rate, liver index, ALT,AST and TBIL content were higher than those in p EGFP-N1 group. With the extension of transgenic time, the expression of fat metabolism-related genes increased, more scarlet fat droplets appeared, and the expression of pMEK1/2 and p-ERKl/2 increased. Conclusion chemokine 2 may increase fat synthesis and promote liver regeneration through MEK/ERK pathway.
【作者单位】：新乡医学院生命科学技术学院;新乡医学院法医学系;
【基金】：河南省高等学校重点科研项目计划(15A180020)
【分类号】：R575

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