乙醇脱氢酶活性对绞窄性肠梗阻大鼠早期肠缺血预测作用的研究

发布时间：2018-10-24 15:25

【摘要】：目的探讨乙醇脱氢酶(ADH)活性对绞窄性肠梗阻大鼠早期肠缺血的预测价值。方法选取成年健康Wistar大鼠104只随机分为对照组(A组,n=8),假手术组(B组,n=32),单纯性肠梗阻(S组,n=32),绞窄性肠梗阻组(I组n=32)。A组大鼠不做任何处理；B组仅开腹翻动肠管后关腹；S组在盲肠末端以上10cm处结扎小肠；I组结扎肠系膜上动脉根部。实验组分别于造模成功后05h、1h、3h、6h下腔静脉取血测定血清中乙醇脱氢酶(ADH)、天冬氨酸氨基转移酶(AST)、丙氨酸转移酶(ALT)水平,并与肝、小肠组织苏木精-伊红染色后损伤病理评分做相关性分析。结果①与A组比较,I组大鼠ADH活性在肠缺血0.5h开始升高,在6h内未见ADH峰值出现。I组在0.5h、1h、3h及6h均高于B组各对应时间点ADH水平,差异有统计学意义(P0.05)；I组在1h、3h及6h高于S组各对应时间点ADH水平,差异有统计学意义(P0.05)；S组与B组各对应时间点ADH比较差异无统计学意义(P0.05)。与B组、S组各对应时间点比较,I组ALT、AST均在6h明显升高,差异有显著统计学意义(P0.05)；②与A组相比,I组各时间点小肠损伤评分差异均有统计学意义(P0.05),I组在3h、6h肝脏损伤病理评分差异有统计学意义(P0.05),B组、S组各时间点小肠、肝脏损伤评分差异均无统计学意义(P0.05)。③I组ADH、ALT、AST水平与小肠、肝脏损伤病理评分均呈正相关(r=0.696～0.795,P0.05)。结论ADH水平在绞窄性肠梗阻早期即开始升高,与小肠损伤程度呈正相关,可能成为预测早期肠绞窄的指标。
[Abstract]:Objective to investigate the predictive value of (ADH) activity of ethanol dehydrogenase in early intestinal ischemia in rats with strangulated intestinal obstruction. Methods 104 healthy adult Wistar rats were randomly divided into three groups: control group (group A, n = 8), sham operation group (group B, n = 32), simple intestinal obstruction (group S, n = 32) and strangulated intestinal obstruction group (n = 32). A). Group S ligated the small intestine at 10cm above the caecum and group I ligated the root of superior mesenteric artery. The serum (ADH), aspartate aminotransferase (ADH),) and alanine transferase (AST),) (ALT) levels in the serum of the experimental group were measured at 0 5 h, 1 h, 3 h and 6 h, respectively, and were compared with those of the liver. The pathological score of small intestine injury after hematoxylin-eosin staining was analyzed. Results 1Compared with group A, the activity of ADH in group I began to increase at 0.5 h after intestinal ischemia, and no peak value of ADH appeared within 6 h. The level of ADH in group I was significantly higher than that in group B at 1 h and 6 h (P0.05). The level of ADH in group I was higher than that in group S at 3h and 6h, and the difference was statistically significant (P0.05). There was no significant difference in ADH between); S group and group B (P0.05). Compared with group B and group S, ALT,AST in group I increased significantly at 6h (P0.05). 2Compared with group A, the small intestine injury scores of group I were significantly different at each time point (P0.05), I group at 3 hours and 6 hours after liver injury) (P0.05), B group, S group at each time point). There was no significant difference in liver injury score (P0.05). 3in group I, there was a positive correlation between ADH,ALT,AST level and small intestine and pathological score of liver injury (r = 0.696 ~ 0.795P, P 0.05). Conclusion the level of ADH increases in the early stage of strangulated intestinal obstruction, and is positively correlated with the degree of intestinal injury, which may be a predictor of early intestinal strangulation.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R574.2

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