Apelin-13对急性坏死性胰腺炎大鼠胰腺组织中自噬的影响

发布时间：2018-12-27 08:15

【摘要】：目的:探讨Apelin-13对大鼠急性坏死性胰腺炎(Acute necrotic pancreatitis,ANP)自噬相关蛋白LC3-II、Beclin-1的影响。方法:取健康雄性SD大鼠54只,将它们随机分为C组(对照组)、P组(ANP组)、S组(ANP+Apelin-13治疗组),各组18只。C组为假手术组,P组SD大鼠以5%的牛黄胆酸钠胆胰管逆行推注制造急性坏死性胰腺炎模型,S组是造模后5min给予Apelin-13尾静脉注射干预组。P组和S组在建立急性坏死性胰腺炎模型后5min,分别尾静脉注射等量生理盐水和Apelin-13(0.1ug/g)。每组大鼠按不同的时相点分别于术后第3,6,12h麻醉后腹主动脉采血,采取血清检测淀粉酶;并取一部分胰腺组织用4%的甲醛固定,做常规病理切片和HE染色;另一部分用Western-Blot检测自噬有关蛋白LC3-II、Beclin-1的表达情况。结果:1.胰腺病理学评分:(1)P组、S组和C组之间各时间点的病理损伤评分差异有着显著性(P0.05);(2)P组损伤评分明显增加,随时间增加损伤程度增加,各时间点差异有显著性(P0.05):(3)S组较P组各时间点的病理损伤评分有所减轻(P0.05)。2.血清淀粉酶的变化:(1)C组的各个时间点大鼠血清淀粉酶未见明显差异(P0.05)。(2)各个时点P组大鼠血清淀粉酶较C组(对照组)显著升高(P0.05);与P组相比较,S组大鼠各时间点的血清淀粉酶有明显降低(P0.05)。3.Western-Blot检测LC3-II,Beclin-1蛋白的表达结果:与C组相比,在大鼠发生ANP 3h,6h,12h后P组自噬相关蛋白LC3-II、Beclin-1的蛋白表达均有着显著增加(P0.05);然而与P组相比,S组各时间点自噬相关蛋白LC3-II和Beclin-1的蛋白表达水平出现明显降低(P0.05)。结论:1、急性坏死性胰腺炎时自噬的表达是增加的,且这种自噬的表达会随着病理损伤程度加重而增加。2、Apelin-13可以明显抑制急性坏死性胰腺炎后大鼠胰腺组织细胞自噬相关蛋白Beclin-1,LC3-II的表达。
[Abstract]:Aim: to investigate the effect of Apelin-13 on autophagy associated protein (LC3-II,Beclin-1) in (Acute necrotic pancreatitis,ANP of rats with acute necrotizing pancreatitis. Methods: Fifty-four healthy male SD rats were randomly divided into group C (control group,), P group (ANP group), S group (ANP Apelin-13 treatment group), 18 rats in each group. Acute necrotizing pancreatitis was induced by retrograde injection of 5% sodium taurocholate into the biliary and pancreatic duct of SD rats in group P. the rats in group S were treated with Apelin-13 caudal vein injection after model making, and group P and group S were treated with Apelin-13 for 5 min after the establishment of acute necrotizing pancreatitis. The same amount of saline and Apelin-13 (0.1ug/g) were injected into caudal vein respectively. The blood samples of abdominal aorta were collected from each group according to different time phase points at 6h after anesthesia, and serum amylase was detected, and some pancreatic tissues were fixed with 4% formaldehyde for routine pathological sections and HE staining. In the other part, the expression of autophagy related protein LC3-II,Beclin-1 was detected by Western-Blot. Results: 1. Pancreatic pathological score: (1) there was significant difference in pathological injury score between P group, S group and C group at each time point (P0.05); (2) the injury score of group P increased significantly, and the degree of injury increased with the increase of time. There was significant difference at each time point (P0.05): (3). The pathological injury score of group S was lighter than that of group P (P0.05). The changes of serum amylase: (1) there was no significant difference in serum amylase in group C (P0.05). (2). The serum amylase in group P was significantly higher than that in group C (P0.05). Compared with group P, serum amylase in group S was significantly lower than that in group P (P0.05). The expression of LC3-II,Beclin-1 protein was detected by 3.Western-Blot: compared with group C, ANP occurred in rats for 3 h or 6 h. After 12 hours, the protein expression of autophagy associated protein LC3-II,Beclin-1 was significantly increased in P group (P0.05). However, compared with P group, the expression levels of autophagy associated protein LC3-II and Beclin-1 in S group were significantly decreased at each time point (P0.05). Conclusion: 1. The expression of autophagy was increased in acute necrotizing pancreatitis, and the expression of autophagy increased with the severity of pathological injury. Apelin-13 could significantly inhibit the expression of autophagy associated protein (Beclin-1,LC3-II) in pancreatic tissue of rats after acute necrotizing pancreatitis.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R576

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