重组人小肠三叶因子对大鼠溃疡性结肠炎的治疗作用

发布时间：2019-02-12 19:27

【摘要】：目的观察重组人小肠三叶因子(recombinated mutant of human intestinal trefoil factor,rh ITF)对大鼠溃疡性结肠炎(ulcerative colitis,UC)的治疗作用,并探讨其可能机制。方法 11~12周龄、体质量(200±20)g的SD雄性大鼠32只,按随机数字表法分为4组:正常组(正常饲养)、模型组(灌胃蒸馏水)、美沙组(灌胃浓度为0.016 g/m L的美沙拉秦缓释颗粒剂溶液)、肠三叶因子(intestinal trefoil factor,ITF)组(灌胃浓度为0.05 mg/m L的rh ITF溶液)(n=8)。造模并治疗12 d后,进行DAI评分,观察结肠病理改变,ELISA法检测大鼠血清及结肠组织中转化生长因子-β1(transforming growth factor-β1,TGF-β1)、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的含量。结果 ITF组:DAI指数在治疗后第4天(1.75±0.59)、8天(0.83±0.31)、12天(0.59±0.30)为逐渐降低趋势;溃疡愈合程度良好,结直肠病灶、病理形态均有明显改善,均优于其它组;血清、肠组织中TGF-β1含量为(414.07±54.24)、(418.60±19.74)ng/L,TNF-α含量为(44.89±2.54)、(48.67±7.12)ng/L,与美沙组、模型组和正常组比较差异显著(P0.05)。结论 rh ITF可以显著改善UC大鼠症状、病理形态,通过上调抗炎因子TGF-β1、抑制促炎因子TNF-α的水平,减轻UC大鼠模型受损肠组织的炎症反应。
[Abstract]:Objective to observe the therapeutic effect of recombinant human intestinal trefoil factor (recombinated mutant of human intestinal trefoil factor,rh ITF) on ulcerative colitis (ulcerative colitis,UC) in rats and to explore its possible mechanism. Methods Thirty two male SD rats of 11 ~ 12 weeks old weighing (200 卤20) g were randomly divided into 4 groups: normal group (normal feeding), model group (intragastric distilled water). Methadone (0.016 g / mL) and intestinal trefoil factor (intestinal trefoil factor,ITF) group (rh ITF solution, 0.05 mg/m / L) (n = 8). After 12 days of model making and treatment, DAI score was used to observe the pathological changes of colon, transforming growth factor- 尾 1 (transforming growth factor- 尾 1 TGF- 尾 1 and tumor necrosis factor- 伪 (tumor necrosis factor- 伪 in serum and colon tissue of rats were detected by ELISA method. The content of TNF- 伪. Results in ITF group, DAI index decreased gradually on day 4 (1.75 卤0.59), day 8 (0.83 卤0.31), day 12 (0.59 卤0.30). The healing degree of ulcers was good, the colorectal lesions and histopathology were improved obviously, which were superior to the other groups. The contents of TGF- 尾 1 in serum and intestinal tissue were (414.07 卤54.24), (卤19.74) ng/L,TNF- 伪, (44.89 卤2.54), (, 48.67 卤7.12) ng/L, and (44.89 卤2.54), () ng/L, respectively. There was significant difference between model group and normal group (P0.05). Conclusion rh ITF can significantly improve the symptoms and pathological morphology of UC rats. By upregulating TGF- 尾 1 and inhibiting the level of proinflammatory factor TNF- 伪, the inflammatory response of injured intestinal tissue in UC rat model can be alleviated.
【作者单位】：辽宁中医药大学附属医院肛肠科;辽宁中医药大学基础医学院;
【基金】：国家自然科学基金(81202688)
【分类号】：R574.62

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