舒芬太尼预处理在大鼠肝脏缺血再灌注损伤的作用及对p-JNK表达的影响

发布时间：2019-04-20 14:10

【摘要】：目的观察舒芬太尼预处理对大鼠肝脏缺血再灌注损伤中JNK及p-JNK表达的影响,探讨其对肝缺血再灌注损伤保护作用的机制。方法健康SD大鼠40只,雌雄不拘,体重200~280 g,采用随机数字表法将其分为5组(n=8):假手术组(C组)、肝缺血再灌注组(IR组)、5μg/kg舒芬太尼预处理组(SF组)、JNK特异性阻断剂SP600125组(SP组)和溶解剂二甲基亚砜组(DMSO组),各组在灌注4 h时取材。采腹主动脉血测定各组大鼠血清中ALT和AST活性;处死大鼠,切取肝组织样本,检测肝组织丙二醛(MDA)和超氧化物歧化酶(SOD)的变化;HE染色光镜下观察肝组织病理学改变;Western blot法测定肝右叶组织JNK和p-JNK的表达水平。结果肝脏缺血30 min,再灌注4 h后,IR、SF、SP及DMSO组血清ALT、AST和肝组织MDA较C组均升高(P0.05)。SF组及SP组血清ALT、AST及肝组织MDA水平较IR组均明显下降,肝组织SOD水平较IR组明显升高(P0.05),病理学损伤较IR组明显减轻;IR组较SF组p-JNK表达明显升高(P0.05);应用JNK特异性阻断剂SP600125,肝损伤减轻,且与SF组相比,各组指标差异无统计学意义(P0.05)。结论舒芬太尼预处理可减轻大鼠肝缺血再灌注损伤,其机制可能是通过抑制JNK通路,减少JNK磷酸化蛋白的表达,减轻炎症因子的产生,从而对大鼠肝脏缺血再灌注损伤起保护作用。二甲基亚砜对大鼠肝脏缺血再灌注损伤无保护作用。
[Abstract]:Aim to observe the effect of sufentanil preconditioning on the expression of JNK and p-JNK in rat liver ischemia-reperfusion injury and to explore the protective mechanism of sufentanil on hepatic ischemia-reperfusion injury. Methods 40 healthy SD rats were randomly divided into 5 groups (n = 8): sham operation group (C group), liver ischemia reperfusion group (IR group), 5 渭 g / kg sufentanil preconditioning group (SF group). The JNK specific blocker SP600125 group (SP group) and the soluble agent dimethyl sulfoxide group (DMSO group) were taken at 4 h after perfusion. The activities of ALT and AST in serum of rats in each group were measured by abdominal aorta blood, the liver tissue samples were harvested and the changes of malondialdehyde (MDA) and superoxide dismutase (SOD) (SOD) in liver tissue were detected, and the pathological changes of liver tissue were observed under light microscope with HE staining. The expression levels of JNK and p-JNK in right lobe of liver were measured by Western blot. Results after 30 min, reperfusion for 4 h, the levels of serum ALT,AST and hepatic MDA in IR,SF,SP and DMSO groups were significantly higher than those in C group (P 0.05). The levels of ALT,AST in serum and MDA in liver tissue in SP group and). SF group were significantly lower than those in IR group. The level of SOD in liver tissue was significantly higher than that in IR group (P0.05), and the pathological injury was significantly less than that in IR group. The expression of p-JNK in IR group was significantly higher than that in SF group (P0.05), the liver injury was alleviated by JNK specific blocker SP600125, and there was no significant difference compared with SF group (P0.05). Conclusion sufentanil pretreatment can alleviate hepatic ischemia-reperfusion injury in rats by inhibiting the JNK pathway, reducing the expression of JNK phosphorylation protein and reducing the production of inflammatory factors. Therefore, it can protect rat liver from ischemia-reperfusion injury. Dimethyl sulfoxide has no protective effect on hepatic ischemia reperfusion injury in rats.
【作者单位】：石河子大学医学院第一附属医院麻醉科;
【分类号】：R575

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