Tempol对大鼠急性重症胰腺炎的疗效及对JNK通路活化的影响

发布时间：2019-04-24 16:51

【摘要】：目的观察Tempol对大鼠急性重症胰腺炎的疗效及对JNK通路的影响。方法 90只大鼠随机分为空白组10只、模型组40只和Tempol组40只。模型组和Tempol组腹腔注射L-精氨酸建立大鼠急性重症胰腺炎模型,空白组注射等量生理盐水;Tempol组于模型建立前5 min按100 mg/kg血管注射Tempol,模型组则注射等量生理盐水。检测模型建立后3、12、24、36 h血清淀粉酶、底物磷酸化c-jun蛋白、丙二醛(MDA)水平。结果空白组血清淀粉酶水平为(1 082.9±523.3)U/L,模型组和Tempol组各时间点淀粉酶水平均高于空白对照组(P0.01),而Tempol组各时间点淀粉酶水平均显著低于模型组(P0.01);空白组磷酸化c-jun蛋白水平为0.33±0.12,Tempol组各时间点磷酸化c-jun蛋白水平均显著低于模型组(P0.01);空白组组织中MDA水平为(5.13±0.48)mmol/L,Tempol组各时间点MDA水平均显著低于模型组(P0.01)。结论 Tempol可通过抑制JNK的活化治疗急性重症胰腺炎。
[Abstract]:Objective to observe the therapeutic effect of Tempol on severe acute pancreatitis (SAP) in rats and its effect on JNK pathway. Methods 90 rats were randomly divided into blank group (n = 10), model group (n = 40) and Tempol group (n = 40). The model group and Tempol group were intraperitoneally injected with L-arginine to establish the model of acute severe pancreatitis, the blank group was injected with the same amount of normal saline, and the Tempol group was injected with the same amount of saline 5 min before the establishment of the model. The Tempol, model group was injected with 100 mg/kg blood vessel. The levels of serum amylase, substrate phosphorylated c-jun protein and malondialdehyde (MDA) were measured 3,12,24,36 hours after the establishment of the model. Results the serum amylase level in the blank group was (1 082.9 卤523.3) U / L, and the amylase level in the model group and Tempol group was higher than that in the blank control group at each time point (P0.01). The amylase level in Tempol group was significantly lower than that in model group (P0.01). The level of phosphorylated c-jun protein in blank group was 0.33 卤0.12, and the level of phosphorylated c-jun protein in Tempol group was significantly lower than that in model group (P0.01). The level of MDA in the blank group was (5.13 卤0.48) mmol/L,Tempol, and the level of MDA in the control group was significantly lower than that in the model group (P0.01). Conclusion Tempol can inhibit the activation of JNK in the treatment of severe acute pancreatitis.
【作者单位】：浙江省立同德医院普外科;
【基金】：浙江省医药卫生一般研究计划项目(编号:2012KYB041)
【分类号】：R576

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