赶黄草对大鼠酒精性脂肪肝的保护作用及机制

发布时间：2019-08-11 13:13

【摘要】：目的探讨赶黄草对大鼠酒精性脂肪肝的保护作用及机制。方法随机选择140例大鼠,喂以1.5%的硫酸亚铁饲料进行饲养,并进行灌胃乙醇制作酒精性脂肪肝大鼠模型。将所有大鼠随机分为正常对照组、模型组、赶黄草提取物高剂量、低剂量组、槲皮素高剂量、低剂量组、硫普罗宁组共七组,用药大鼠均灌胃给药,1次/d,连用6 w。计算肝脏系数,并行肝组织油红O染色,于光镜下观察肝脏脂肪浸润情况,对比肝细胞脂滴面积,并检测谷丙转氨酶(ALT)、谷草转氨酶(AST)、血清总胆固醇(TC)及甘油三酯(TG)含量。结果 1相比正常对照组,模型组大鼠反应迟钝,体重明显减轻,肝脏指数明显增加(P0.05);相比模型组,各用药组大鼠体重增加显著,肝脏指数明显下降(P0.05)。2油红O染色脂滴半定量分析后显示,赶黄草提取物各剂量组同模型组比较均有明显差异(P0.05)。3相比正常对照组,模型组血清ALT、AST、TC、TG水平均增加(P0.05);相比模型组,各用药组血清ALT、AST、TC、TG水平均降低(P0.05);相比槲皮素各组,赶黄草各提取物组ALT、AST、TC、TG水平均降低(P0.05)。结论赶黄草对大鼠酒精性脂肪肝有明显保护作用,与其中含有的槲皮素及其他化学成分有关。
[Abstract]:Objective to investigate the protective effect and mechanism of Elaeagnus angustifolia on alcoholic fatty liver in rats. Methods 140 rats were randomly selected and fed with 1.5% ferrous sulfate feed, and the rat model of alcoholic fatty liver was established by intragastric administration of ethanol. All the rats were randomly divided into seven groups: normal control group, model group, high dose, low dose group, quercetin high dose group, low dose group and tiopronin group. The rats were given intragastrically once a day for 6 weeks. The liver coefficient was calculated and the liver tissue was stained with oil red O. The fat infiltration of liver was observed under light microscope, the area of lipid droplets in hepatocytes was compared, and the contents of total cholesterol (TC) and triglyceride (TG) in serum of glutamic-pyruvic transaminase (ALT), glutamic oxaloacetic transaminase (AST), were measured. Results 1 compared with the normal control group, the rats in the model group were slow to respond, the body weight was significantly reduced, and the liver index was significantly increased (P 0.05). Compared with the model group, the body weight of the rats in each treatment group increased significantly, and the liver index decreased significantly (P 0.05). 2 after semi-quantitative analysis of oil red O staining lipid droplets, there was significant difference between each dose group and the model group (P 0.05). 3 compared with the normal control group, the serum ALT,AST,TC,TG level in the model group was increased (P 0.05). Compared with the model group, the serum ALT,AST,TC,TG level of each drug group was decreased (P 0.05), and the ALT,AST,TC,TG level of each extract group was lower than that of quercetin group (P 0.05). Conclusion Elaeagnus angustifolia has obvious protective effect on alcoholic fatty liver in rats, which is related to quercetin and other chemical constituents.
【作者单位】：泸州医学院附属中医医院静脉用药调配中心;
【分类号】：R575.5

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