MDSCs与CXCR4/CXCL12轴在非酒精性脂肪性肝炎小鼠中的作用及其机制研究
发布时间：2021-07-13 13:17
第一部分构建CDAA饮食诱导的小鼠NASH模型目的:构建CDAA饮食诱导的NASH小鼠模型,为进一步的深入研究做基础。方法:选用6-8周龄雄性SPF级C57BL小鼠,经适应性喂养1周后分为正常普通饮食NF组、CDAA模型组、CSAA对照组直到第12周末结束。造模结束时,留取血清检测生化指标:丙氨酸氨基转化酶（ALT）、门冬氨酸氨基转移酶（AST）、甘油三酯（TG）、尿素氮（BUN）、血糖（Blood Glucose）、总胆固醇（T-CHO）、总胆红素（TBIL）的水平;肝组织经石蜡包埋HE染色,镜下观察小鼠肝组织病理变化,天狼猩红染色评价NASH模型纤维化程度。并应用NAFLD活动度积分（NAFLD activity score,NAS）评分判断NASH模型程度;ELISA方法检测正常组、对照组、模型组外周血炎性因子PGE2、IL-6、INF-γ水平。结果:（1）经纯化CDAA模型饮食、CSAA对照饮食组喂养小鼠12周末其平均体重,肝指数与正常组无显著差异（P>0.05）。模型组小鼠肝脏色泽变黄,边缘较钝,质地较硬,弹性差。模型组外周血清ALT、AST、Glucose较正常组均明...
【文章来源】：山西医科大学山西省
【文章页数】：150 页
【学位级别】：博士
【部分图文】：
实验小鼠不同时间点体重的变化
图 2-1 实验小鼠不同时间点体重的变化20 23.4 ± 0.93 24.6 ± 1.12 26.1 ± 1.35 26.5 ± 1.64 27.4 ± 1.87 28.6 ± 1.93 30.1±2.02 31.2 ± 2.1120 23.42 ± 1.27 24.88 ± 1.39 26.23 ± 1.49 27.50 ± 1.70 28.49 ± 2.40 29.34 ± 2.99 30.36±3.48 29.87 ± 3.3720 23.41 ± 0.90 24.28 ± 1.23 25.63 ± 1.93 27.00 ± 1.67 28.05 ± 1.83 29.05 ± 2.02 29.72±2.33 29.72 ± 2.33
图 2-1 实验小鼠不同时间点体重的变化20 23.4 ± 0.93 24.6 ± 1.12 26.1 ± 1.35 26.5 ± 1.64 27.4 ± 1.87 28.6 ± 1.93 30.1±2.02 31.2 ± 2.1120 23.42 ± 1.27 24.88 ± 1.39 26.23 ± 1.49 27.50 ± 1.70 28.49 ± 2.40 29.34 ± 2.99 30.36±3.48 29.87 ± 3.3720 23.41 ± 0.90 24.28 ± 1.23 25.63 ± 1.93 27.00 ± 1.67 28.05 ± 1.83 29.05 ± 2.02 29.72±2.33 29.72 ± 2.33
【参考文献】：
期刊论文
[1]Hepatic immune tolerance induced by hepatic stellate cells[J]. Ching-Chuan Hsieh,Chien-Hui Hung,Lina Lu,Shiguang Qian. World Journal of Gastroenterology. 2015(42)
[2]Emerging roles of myeloid derived suppressor cells in hepatic inflammation and fibrosis[J]. Linda Hammerich,Frank Tacke. World Journal of Gastrointestinal Pathophysiology. 2015(03)
[3]Prevalence of fatty liver disease and its risk factors in the population of South China[J]. Yong-Jian Zhou,Yu-Yuan Li,Min-Hu Chen,Pin-Jin Hu,Department of Gastroenterology,The First Affiliated Hospital,Sun Yat-Sen University of Medical Sciences,Guangzhou 510080,Guangdong Province ,China Yong-Jian Zhou,Yu-Yuan Li,Yu-Qiang Nie,Sheng-Li Shi,Department of Gastroenterology and Hepatology,First Municipal People’s Hospital of Guangzhou,Guangzhou Medical College,Guangzhou 510180,Guangdong Province,China Jin-Xiang Ma,Department of Prevention Medicine,Guangzhou Medical College,Guangzhou 510182,Guangdong Province,China Lun-Gen Lu,Department of Gastroenterology,Renji Hospital,Shanghai Institute of Digestive Disease,Shanghai Jiaotong University School of Medicine,Shanghai 200001,China. World Journal of Gastroenterology. 2007(47)
本文编号：3282133
【文章来源】：山西医科大学山西省
【文章页数】：150 页
【学位级别】：博士
【部分图文】：
实验小鼠不同时间点体重的变化
图 2-1 实验小鼠不同时间点体重的变化20 23.4 ± 0.93 24.6 ± 1.12 26.1 ± 1.35 26.5 ± 1.64 27.4 ± 1.87 28.6 ± 1.93 30.1±2.02 31.2 ± 2.1120 23.42 ± 1.27 24.88 ± 1.39 26.23 ± 1.49 27.50 ± 1.70 28.49 ± 2.40 29.34 ± 2.99 30.36±3.48 29.87 ± 3.3720 23.41 ± 0.90 24.28 ± 1.23 25.63 ± 1.93 27.00 ± 1.67 28.05 ± 1.83 29.05 ± 2.02 29.72±2.33 29.72 ± 2.33
图 2-1 实验小鼠不同时间点体重的变化20 23.4 ± 0.93 24.6 ± 1.12 26.1 ± 1.35 26.5 ± 1.64 27.4 ± 1.87 28.6 ± 1.93 30.1±2.02 31.2 ± 2.1120 23.42 ± 1.27 24.88 ± 1.39 26.23 ± 1.49 27.50 ± 1.70 28.49 ± 2.40 29.34 ± 2.99 30.36±3.48 29.87 ± 3.3720 23.41 ± 0.90 24.28 ± 1.23 25.63 ± 1.93 27.00 ± 1.67 28.05 ± 1.83 29.05 ± 2.02 29.72±2.33 29.72 ± 2.33
【参考文献】：
期刊论文
[1]Hepatic immune tolerance induced by hepatic stellate cells[J]. Ching-Chuan Hsieh,Chien-Hui Hung,Lina Lu,Shiguang Qian. World Journal of Gastroenterology. 2015(42)
[2]Emerging roles of myeloid derived suppressor cells in hepatic inflammation and fibrosis[J]. Linda Hammerich,Frank Tacke. World Journal of Gastrointestinal Pathophysiology. 2015(03)
[3]Prevalence of fatty liver disease and its risk factors in the population of South China[J]. Yong-Jian Zhou,Yu-Yuan Li,Min-Hu Chen,Pin-Jin Hu,Department of Gastroenterology,The First Affiliated Hospital,Sun Yat-Sen University of Medical Sciences,Guangzhou 510080,Guangdong Province ,China Yong-Jian Zhou,Yu-Yuan Li,Yu-Qiang Nie,Sheng-Li Shi,Department of Gastroenterology and Hepatology,First Municipal People’s Hospital of Guangzhou,Guangzhou Medical College,Guangzhou 510180,Guangdong Province,China Jin-Xiang Ma,Department of Prevention Medicine,Guangzhou Medical College,Guangzhou 510182,Guangdong Province,China Lun-Gen Lu,Department of Gastroenterology,Renji Hospital,Shanghai Institute of Digestive Disease,Shanghai Jiaotong University School of Medicine,Shanghai 200001,China. World Journal of Gastroenterology. 2007(47)
本文编号：3282133
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