中耳胆脂瘤中MMP-1、MMP-2的表达和相关性研究

发布时间：2017-12-31 05:32

本文关键词：中耳胆脂瘤中MMP-1、MMP-2的表达和相关性研究　出处：《新疆医科大学》2013年硕士论文　论文类型：学位论文

【摘要】：目的：研究两种基质金属蛋白酶（MMP-1和MMP-2）在中耳胆脂瘤上皮组织中的表达与侵袭破坏的关系，并探讨二者的相关性，从分子水平了解胆脂瘤的发病机制。方法：采用免疫组化法检测39例经病理切片证实为胆脂瘤型中耳炎患者不同部位的中耳胆脂瘤上皮标本中的MMP-1和MMP-2表达，，根据患者听力学检查分为轻度聋、中度聋、中重度聋、重度聋、极重度聋；根据术中所见将胆脂瘤侵袭情况分为Ⅰ度、Ⅱ度、Ⅲ度和Ⅳ度，结合患者临床资料及胆脂瘤取材部位等进行统计分析。结果：不同的听力损失等级、胆脂瘤侵袭情况的胆脂瘤上皮组织中MMP-1、MMP-2的阳性表达的差异有统计学意义（P＜0.05），MMP-1和MMP-2的表达呈正相关（r=0.831，P＜0.05）。性别、年龄，取材部位对MMP-1和MMP-2的表达均无统计学意义（P＞0.05）。结论：1．MMP-1和MMP-2在中耳胆脂瘤上皮中均有高表达，表明二者在胆脂瘤上皮的增殖及骨质破坏的过程中都发挥重要作用。2．MMP-1和MMP-2在中耳胆脂瘤组织中随着周围骨质破坏程度的范围扩大和患者听力损失程度的加重，二者表达量也随之增高，提示二者在胆脂瘤上皮对骨质的破坏行为和听力损害中起非常重要的作用。3．MMP-1和MMP-2之间在中耳胆脂瘤上皮组织中的表达呈正相关，提示二者在胆脂瘤组织中的增殖和侵袭行为中可能有协同作用。
[Abstract]:Objective : To study the relationship between the expression of MMP - 1 and MMP - 2 in the epithelial tissue of middle ear cholesterin by two kinds of matrix metalloproteinases ( MMP - 1 and MMP - 2 ) . The expression of MMP - 1 and MMP - 2 was detected by immunohistochemistry . Results : The expression of MMP - 1 and MMP - 2 was positively correlated with the expression of MMP - 1 and MMP - 2 ( r = 0.831 , P < 0.05 ) . The expression of MMP - 1 and MMP - 2 was not statistically significant ( P > 0.05 ) . Conclusion : 1 . MMP - 1 and MMP - 2 were highly expressed in the epithelium of middle ear cholesterin , indicating that both MMP - 1 and MMP - 2 play an important role in the proliferation and destruction of tumor epithelial cells . The expression of MMP - 1 and MMP - 2 increased with the extent of peripheral bone destruction and the degree of hearing loss , suggesting that both of MMP - 1 and MMP - 2 play a very important role in the destruction of bone and hearing impairment . The expression of MMP - 1 and MMP - 2 was positively correlated with the expression of MMP - 1 and MMP - 2 in the epithelial tissue of the middle ear .

【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R764.21

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