姜黄素对人喉癌Hep-2细胞放疗敏感性的实验研究

发布时间：2018-01-05 22:16

本文关键词：姜黄素对人喉癌Hep-2细胞放疗敏感性的实验研究　出处：《中医临床研究》2013年16期 　论文类型：期刊论文

【摘要】：目的:探讨姜黄素对人喉癌Hep-2细胞的放疗敏感性影响。方法:不同浓度姜黄素作用人喉癌Hep-2细胞24h、48h、72h后,加入DMSO或SDS使用MTT法检测姜黄素毒性;克隆形成实验观察其对放射敏感性的影响。紫杉醇与20μmol/L姜黄素联合作用Hep-2细胞24h。流式细胞术检测姜黄素诱导细胞的凋亡率、细胞周期分布。结果:姜黄素对人喉癌Hep-2细胞有明显抑制作用,存在剂量和时间依赖;20μmol/L姜黄素可降低放射后Hep-2细胞的克隆形成率。联合用药诱导细胞凋亡作用增强。姜黄素及姜黄素联合辐射组的细胞周期阻滞在辐射敏感时相G2/M期。结论:姜黄素可提高人喉癌Hep-2细胞放射敏感性,可能与其引起瘤细胞周期阻滞,诱导细胞凋亡有关。紫杉醇与姜黄素联合化疗有减毒增效作用。
[Abstract]:Objective: to investigate the effect of curcumin on the radiosensitivity of human laryngeal carcinoma Hep-2 cells. Methods: human laryngeal carcinoma Hep-2 cells were treated with curcumin for 24 h or 48 h for 72 h. The toxicity of curcumin was detected by MTT with DMSO or SDS. The effects of paclitaxel and 20 渭 mol/L curcumin on the radiosensitivity of Hep-2 cells were observed. Flow cytometry was used to detect the apoptosis rate of Hep-2 cells induced by curcumin. Results: curcumin had obvious inhibitory effect on human laryngeal carcinoma Hep-2 cells in a dose-and time-dependent manner. 20 渭 mol/L curcumin could reduce the clone formation rate of Hep-2 cells after irradiation, and the apoptosis induced by combined use of curcumin and curcumin was enhanced. The cell cycle arrest of curcumin combined with curcumin radiation group was sensitive to radiation. Conclusion:. Curcumin can enhance radiosensitivity of human laryngeal carcinoma Hep-2 cells. It may be related to cell cycle arrest and apoptosis induced by paclitaxel combined with curcumin.
【作者单位】：河南科技大学第一附属医院;
【基金】：2012年河南省科技厅科技攻关项目,项目编号:122102310172
【分类号】：R285.5
【正文快照】： 最近研究显示姜黄素对喉癌细胞Hep-2有明显的抑制作用,呈剂量依赖性;流式细胞仪分析阻止G1期细胞向S期进展,进而促进Hep-2凋亡[1],但较少发现关于放射线对姜黄素作用后喉癌Hep-2细胞的生长影响的实验研究。本文以人喉癌细胞系Hep-2为对象,研究姜黄素对肿瘤放射敏感性的影响。

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