糖基化终末产物对体外培养牛眼小梁细胞氧化应激及凋亡的影响

发布时间：2018-01-08 16:38

本文关键词：糖基化终末产物对体外培养牛眼小梁细胞氧化应激及凋亡的影响　出处：《眼科新进展》2014年07期 　论文类型：期刊论文

【摘要】：目的通过观察糖基化终末产物(advanced glycation end products,AGE)对体外培养牛眼小梁细胞凋亡的影响,研究AGE与原发性开角型青光眼(primary open angle glaucoma,POAG)之间的关系,进一步探讨其发病机制。方法体外培养牛眼小梁细胞,通过形态学观察和神经元特异性烯醇化酶染色对培养的细胞进行鉴定。将第3代小梁细胞接种于6孔培养板,在培养基中加入不同浓度(0μg·mL-1、50μg·mL-1、100μg·mL-1、200μg·mL-1)的AGE-BSA培养96 h。终浓度(200μg·mL-1)的AGE-BSA培养液处理细胞不同时间(48 h、72 h、96h)。应用流式细胞仪检测小梁细胞凋亡率;活性氧荧光探针2’,7’-二氯荧光黄双乙酸盐检测细胞内活性氧(ROS)水平。结果 50μg·mL-1、100μg·mL-1、200μg·mL-1AGE-BSA作用96h后细胞凋亡率分别为(5.60±0.25)%、(9.57±0.08)%、(17.68±0.21)%,与对照组(0μg·mL-1AGE-BSA)细胞凋亡率(4.45±0.12)%相比均明显增高,且差异均有统计学意义(均为P0.05);200μg·mL-1AGE-BSA作用细胞48 h、72 h、96 h后凋亡率分别为(10.51±0.28)%、(13.47±0.42)%、(17.68±0.21)%,与对照组相比也均明显增高,差异均有统计学意义(均为P0.05)。与对照组比较,AGE-BSA处理后细胞内ROS水平显著提高,差异有统计学意义(P0.05),BSA组差异无统计学意义(P0.05)。结论在体外培养的条件下,AGE可能通过刺激牛眼小梁细胞产生大量ROS介导小梁细胞凋亡。
[Abstract]:Objective to investigate the effect of advanced glycation end products (ages) on apoptosis of bovine trabecular meshwork cells in vitro. To investigate the relationship between AGE and primary open angle glaucoma (primary open angle glaucoma gaunt). Methods Bovine trabecular meshwork cells were cultured in vitro. The cultured cells were identified by morphological observation and neuron-specific enolase staining. The third passage trabecular cells were inoculated into 6-well culture plate, and different concentrations of 0 渭 g 路mL-1 were added to the culture medium. 50 渭 g 路ml -1 and 100 渭 g 路mL-1. The cells were incubated with 200 渭 g 路ml -1 AGE-BSA for 96 h and with the final concentration of 200 渭 g 路ml -1 AGE-BSA for 48 h. The apoptosis rate of trabecular cells was detected by flow cytometry. The level of reactive oxygen species (Ros) was detected by the reactive oxygen species (Ros) probe 2 ~ (2 +) -dichlorofluorescein yellow diacetate. Results 50 渭 g 路mL ~ (-1) 路L ~ (-1) ~ (-1) 渭 g 路mL-1 of reactive oxygen species was detected. After treated with 200 渭 g 路mL-1AGE-BSA for 96 h, the apoptosis rate was 5.60 卤0.25% and 9.57 卤0.08%, respectively. Compared with the control group (0 渭 g 路ml ~ (-1)), the apoptotic rate of AGE-BSAs was significantly higher than that of 4.45 卤0.12%. The difference was statistically significant (P 0.05). The apoptosis rates of cells treated with 200 渭 g 路mL-1AGE-BSA for 48 h and 72 h and 96 h were 10.51 卤0.28 and 13.47 卤0.42%, respectively. Compared with the control group, the difference was statistically significant (P 0.05). The intracellular ROS level was significantly increased after AGE-BSA treatment (P 0.05). Conclusion in vitro culture, age may induce a large number of ROS mediated trabecular cell apoptosis by stimulating bovine trabecular meshwork cells.
【作者单位】：滨州医学院;滨州医学院附属医院;
【分类号】：R775.2
【正文快照】： 原发性开角型青光眼(primary open angle glau-coma,POAG)是一种常见致盲性眼病,大型流行病学研究显示糖尿病为POAG最常见的病因,与POAG发病率呈正相关[1]。糖尿病患者中POAG的发病率为 4%~11%[2],比一般人群(2%)高。糖尿病与POAG间的关系密切,但糖尿病患者并发POAG的发病

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