血管内皮生长因子及白介素8在不同病理类型鼻息肉中表达的对比研究

发布时间：2018-01-18 08:32

本文关键词：血管内皮生长因子及白介素8在不同病理类型鼻息肉中表达的对比研究　出处：《大连医科大学》2010年硕士论文　论文类型：学位论文

【摘要】： 目的：通过观察VEGF及IL-8在以嗜酸性细胞为主及以中性粒细胞为主的鼻息肉中的表达,以分析它们在鼻息肉发病机理中的作用。方法：于2007年6月-2009年12月对在我院行鼻内镜手术的240余例鼻息肉患者的标本进行严格筛选,均为临床资料完整的石蜡包埋组织。标本取材前2周均未应用糖皮质激素和抗组胺类药物。选出以嗜酸性细胞浸润为主及以中性粒细胞浸润为主的鼻息肉标本各32例,其中以嗜酸性细胞浸润为主的男21例,女10例,年龄14-72岁,平均50.5岁；以中性粒细胞浸润为主的男19例,女12例,年龄27-69岁,平均48.3岁；对照组选自单纯鼻中隔偏曲患者的正常中鼻甲粘膜为30例,不伴有过敏性鼻炎、慢性鼻炎、慢性化脓性鼻窦炎。以嗜酸性细胞为主的鼻息肉32例,以中性粒细胞为主的鼻息肉32例及正常中鼻甲粘膜30例。将正常中鼻甲粘膜和鼻息肉用10%福尔马林固定,石蜡包埋,切片。用免疫组织化学方法检测血管内皮生长因子及白介素8在正常中鼻甲粘膜和嗜酸性粒细胞及中性粒细胞鼻息肉中的表达。结果：VEGF及IL-8在正常鼻粘膜均呈弱阳性,即少见棕黄色颗粒,在嗜酸性粒细胞及中性粒细胞鼻息肉中呈阳性或强阳性表达。VEGF在以嗜酸性细胞为主的鼻息肉中呈强阳性表达,在以中性粒细胞为主的鼻息肉中呈阳性表达；IL-8在以中性粒细胞为主的鼻息肉中呈强阳性表达,在以嗜酸性细胞为主的鼻息肉中呈阳性表达。VEGF和IL-8在鼻息肉中的表达呈正相关。结论：VEGF在以嗜酸性细胞为主的鼻息肉中为高表达,IL-8在以中性粒细胞为主的鼻息肉中高表达,VEGF和IL-8在鼻息肉中的表达呈正相关。说明两种病理类型鼻息肉发病机理不同的,VEGF及IL-8在鼻息肉的发病机制中起重要作用。
[Abstract]:Aim: to investigate the expression of VEGF and IL-8 in nasal polyps with eosinophils and neutrophils, and to analyze their role in the pathogenesis of nasal polyps. Methods: from June 2007 to December 2009, the specimens of more than 240 cases of nasal polyps underwent endoscopic sinus surgery in our hospital were strictly screened. No glucocorticoids and antihistamines were used 2 weeks before the specimen was collected. The nasal polyps with eosinophil infiltration and neutrophil infiltration were selected respectively. 32 cases. There were 21 males and 10 females with eosinophil infiltration, aged 14-72 years (mean 50.5 years). There were 19 males and 12 females with neutrophil infiltration, aged 27-69 years (mean 48.3 years). In the control group, 30 cases of normal middle turbinate mucosa were selected from patients with simple deviation of nasal septum, without allergic rhinitis, chronic rhinitis and chronic suppurative sinusitis. 32 cases of nasal polyps with eosinophils, 32 cases with neutrophil polyps and 30 cases with normal middle turbinate mucosa were fixed with 10% formalin. The expression of vascular endothelial growth factor (VEGF) and interleukin-8 (IL-8) in normal middle turbinate mucosa, eosinophil and neutrophil nasal polyps were detected by immunohistochemistry. Results both IL-8 and IL-8 were weakly positive in normal nasal mucosa. Positive or strongly positive expression of VEGF in eosinophil and neutrophil nasal polyps. VEGF was strongly expressed in nasal polyps with mainly eosinophil. Positive expression was found in nasal polyps dominated by neutrophils. The expression of IL-8 was strongly positive in nasal polyps with neutrophil and positive correlation with IL-8 in nasal polyps with eosinophilic cells. Conclusion in nasal polyps with eosinophils, the expression of IL-8 is higher than that in neutrophilic polyps. The positive correlation between the expression of VEGF and IL-8 in nasal polyps suggests that the two pathological types of nasal polyps play an important role in the pathogenesis of nasal polyps.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R765.

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