膜蛋白AN01过表达对人喉鳞状细胞癌Hep-2细胞株生物学特性的影响

发布时间：2018-01-24 09:47

本文关键词： 头颈鳞癌氯离子通道转移　出处：《中国医学科学院学报》2014年01期 　论文类型：期刊论文

【摘要】：目的研究膜蛋白ANO1过表达对人喉鳞状细胞癌Hep-2细胞增殖、剥脱、伸展和迁移的影响。方法以ANO1稳定过表达的Hep-2细胞株作为实验组,空白质粒转染的Hep-2细胞作为对照组,采用MTT法检测细胞增殖活性,细胞剥脱实验检测细胞剥脱能力,细胞伸展实验检测细胞伸展能力,Boyden小室侵袭实验、体外划痕愈合实验和尼氟灭酸阻断氯离子通道实验检测细胞迁移能力。结果 MTT法检测结果显示,实验组与对照组的光密度值差异无统计学意义(P=0.62)。细胞剥脱实验和细胞伸展实验结果显示,实验组的细胞剥脱百分比(P0.0001)和伸展百分比(P0.0001)明显大于对照组。Boyden小室侵袭实验结果显示,实验组的穿膜细胞百分比明显大于对照组(P0.0001);体外划痕愈合实验结果显示,实验组的划痕面积百分比明显小于对照组(P0.0001);尼氟灭酸阻断氯离子通道实验结果显示,实验组的划痕面积百分比明显大于对照组(P0.0001)。结论 ANO1过表达并未加快癌细胞的增殖速度,但却大大增加了头颈鳞癌细胞的移动、伸展和剥脱能力。
[Abstract]:Objective to study the effect of membrane protein ANO1 overexpression on proliferation and exfoliation of human laryngeal squamous cell carcinoma (Hep-2) cells. Methods Hep-2 cells with stable expression of ANO1 were used as experimental group and Hep-2 cells transfected with blank plasmid as control group. The cell proliferation activity was detected by MTT assay, the cell stripping ability was detected by cell stripping assay, and the cell extension ability was detected by cell extension assay. In vitro scratch healing test and nifluronic acid blocking chloride channel assay were used to detect the migration ability of the cells. Results the results of MTT assay showed. There was no significant difference in optical density between the experimental group and the control group. The results of cell stripping test and cell extension test showed that there was no significant difference between the experimental group and the control group. The percentage of cell stripping (P 0.0001) and the percentage of extension (P 0.0001) in the experimental group were significantly higher than those in the control group (P 0.0001). The percentage of perforating cells in the experimental group was significantly higher than that in the control group (P 0.0001). The results of in vitro scratch healing test showed that the percentage of scratch area in the experimental group was significantly lower than that in the control group (P 0.0001). The results showed that the percentage of scratch area in the experimental group was significantly higher than that in the control group (P0.0001). Conclusion the overexpression of ANO1 does not accelerate the proliferation of cancer cells. But it greatly increases the ability to move, stretch, and peel off head and neck squamous cancer cells.
【作者单位】：重庆医科大学附属第一医院颌面外科;
【基金】：重庆市自然科学基金(cstc2012jjA10039) 重庆市卫生局医学科研项目(2011-2-013) 重庆市教委科学技术研究项目(KJ130318)~~
【分类号】：R739.65
【正文快照】： 癌细胞转移是头颈鳞癌治疗失败的主要原因[1]。笔者以往采用基因芯片技术检测了80例头颈鳞癌患者的临床标本,结果发现ANO1高表达与头颈鳞癌转移高度相关[2]。对多株头颈鳞癌细胞株的检测结果亦显示,人喉鳞状细胞癌Hep-2细胞内源性ANO1表达较低。遂将含有ANO1片段的PSG-5质粒转

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