血管内皮生长因子、缺氧诱导因子-1α与阻塞性睡眠呼吸暂停低通气综合征
发布时间：2018-01-26 04:55
本文关键词： 阻塞性睡眠呼吸暂停/低通气综合征 缺氧诱导因子-1α 血管内皮生长因子 基因 逆转录-聚合酶链反应(RT-PCR) 酶联免疫吸附试验(ELISA) 聚合酶链反应-限制性片段长度多态性(PCR-RFLP) 出处：《福建医科大学》2011年硕士论文 论文类型：学位论文
【摘要】：目的:探讨血管内皮生长因子(VEGF)、缺氧诱导因子-1α(HIF-1α)在阻塞性睡眠呼吸暂停/低通气综合征(OSAHS)患者及其心血管并发症发生中的意义。 方法:对48例OSAHS合并心血管疾病患者、52例单纯OSAHS患者和100名体检正常者进行多导睡眠监测,采用酶联免疫吸附试验法对三组对象外周血中的VEGF、HIF-1α水平进行检测,外周血VEGF mRNA表达水平的测定采用逆转录-聚合酶链反应及计算机条带分析,并进行相关性分析,运用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测三组对象VEGF基因+813 C/T位点基因型。 结果:(1)三个组别间血浆HIF-1α浓度、VEGF含量、外周血单个核细胞VEGF mRNA的表达比较差异有统计学意义(P 0.01),两两比较显示单纯OSAHS组高于正常对照组,且差异有统计学意义(P 0.01),OSAHS合并心血管病组高于单纯OSAHS组,差异有统计学意义(P 0.01)。(2)①OASHS患者血浆HIF-1α、VEGF水平以及VEGF mRNA的表达水平与AHI、T-SaO290%、晨起收缩压、晨起舒张压呈不同程度正相关,与minSaO2呈负相关。②HIF-1α与血浆VEGF浓度、VEGF mRNA表达呈正相关。(3)与OSAHS患者相比,正常对照组的CT和TT基因型较多,差异有统计学意义(P 0.01);正常对照组的T等位基因较OSAHS组明显增多(P 0.01)。OSAHS组以及正常对照组CC基因型患者血浆VEGF水平均较CT+TT基因型患者相比均有升高,差异有统计学意义(P 0.05)。 结论:HIF-1α在OSAHS患者外周血中的水平明显升高,OSAHS合并心血管疾病组的升高更加明显,主要通过调节VEGF的水平,参与OSAHS的发病,并可能是OSAHS心血管并发症的重要发病机制之一;VEGF基因+813位点出现T等位基因可能降低OSAHS的易感性。
[Abstract]:Objective: To investigate the significance of vascular endothelial growth factor (VEGF) and hypoxia inducible factor -1 alpha (HIF-1 a) in patients with obstructive sleep apnea hypopnea syndrome (OSAHS) and cardiovascular complications.
Methods: 48 cases of OSAHS patients with cardiovascular disease, 52 cases of OSAHS patients and 100 healthy subjects underwent polysomnography, using enzyme-linked immunosorbent assay method in three groups of VEGF in peripheral blood, detected levels of HIF-1 alpha, determined by polymerase chain reaction and computer reverse transcriptase band analysis the level of expression of VEGF in peripheral blood of mRNA, and the correlation analysis was performed, using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique for the detection of three groups of VEGF gene +813 C/T genotype.
Results: (1) between the three groups of plasma HIF-1 concentration, VEGF content, significant expression of VEGF in peripheral blood mononuclear cells mRNA difference (P 0.01), the 22 comparison shows that pure OSAHS group is higher than the normal control group, and the difference was statistically significant (P 0.01), OSAHS group was higher than that of cardiovascular disease in the OSAHS group, the difference was statistically significant (P 0.01). (2) in the plasma of patients with OASHS HIF-1 alpha, VEGF level and VEGF expression level of mRNA and AHI, T-SaO290%, morning systolic, early diastolic blood pressure was positively related to different degrees, and negatively correlated with minSaO2. The HIF-1 alpha and VEGF concentration in plasma VEGF, mRNA expression was positively correlated. (3) compared with OSAHS patients, CT and TT genotypes were the normal control group, the difference was statistically significant (P 0.01); normal control group T allele significantly increased than group OSAHS (P 0.01).OSAHS group and normal control group in plasma of patients with CC genotype VEGF Compared with the CT+TT genotype, the levels of the patients were all higher than those of the CT+TT genotypes, and the difference was statistically significant (P 0.05).
缁撹:HIF-1伪鍦∣SAHS鎮ｈ，
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