甘氨双唑钠对鼻咽癌移植瘤的时辰放射增敏作用

发布时间：2018-01-28 14:03

本文关键词： 甘氨双唑钠时辰放疗乏氧放射增敏剂 HIF-α γ-HAX　出处：《世界科技研究与发展》2010年01期 　论文类型：期刊论文

【摘要】：目的观察甘氨双唑钠(CMNa)联合时辰放疗对鼻咽癌裸鼠移植瘤的时辰放射增敏作用,并探讨其作用机制。方法将荷瘤裸鼠随机分为3组:放疗组、放疗+CMNa组、空白对照组,每组分3HALO(光照后小时,hours after light onset)、9HALO、15HALO、21HALO四个时辰进行相应处理。测定肿瘤再生长延长时间(regrowth delay time,TGD),绘制生长曲线。用免疫组化法检测各组肿瘤标本中HIF-1α、γ-H2AX和凋亡蛋白的表达。结果通过对各组TGD的比较,以放疗+CMNa组对肿瘤的抑制效果最好。在该组中,TGD:15HALO21HALO9HALO3HALO,15HALO与3HALO的TGD比较有统计学意义;15HALO与3HALO的HIF-1 α、γ-H2AX及凋亡蛋白的表达水平比较有统计学意义。结论甘氨双唑钠联合时辰放疗对鼻咽癌裸鼠移植瘤有明显的时辰放射增敏作用,以15HALO放疗+CMNa组对肿瘤的抑制效果最佳。其机制可能与HIF-1α的表达,DNA双链损伤,凋亡有关。
[Abstract]:Objective to observe the effect of CMNa combined with time radiotherapy on the sensitization of nasopharyngeal carcinoma xenografts in nude mice and to explore its mechanism. Methods Nude mice were randomly divided into three groups: radiotherapy group. CMNa group, blank control group, 3HALO (hours after light onsett) 15HALO in each group. 21HALO was treated at four hours. The tumor growth prolongation time and regrowth delay time were measured. The expression of HIF-1 伪, 纬 -H2AX and apoptotic protein was detected by immunohistochemical method. The tumor inhibition was best in CMNa group with TGD: 15HALO21HALO9HALO3HALO. The TGD of 15HALO was significantly higher than that of 3HALO. HIF-1 伪 of 15HALO and 3HALO. The expression of 纬 -H2AX and apoptotic protein was significantly higher than that of control group. Conclusion Glycosaminodiazole sodium combined with time radiotherapy can enhance the sensitivity of xenografts of nasopharyngeal carcinoma in nude mice. 15HALO radiation group had the best inhibitory effect on tumor, and its mechanism might be related to the double strand damage and apoptosis of HIF-1 伪 expression.
【作者单位】：重庆医科大学基础医学院放射医学教研室;
【分类号】：R739.63
【正文快照】： 1引言鼻咽癌放疗后局部控制欠佳或出现远处转移是其单纯放疗失败的主要原因,而局部控制欠佳的重要原因之一是肿瘤细胞中乏氧细胞的存在。有报道表明,实体肿瘤中乏氧细胞比例约占10%一50%。乏氧细胞的存在降低了肿瘤对射线的敏感性,而乏氧细胞增敏剂是解决肿瘤内乏氧放射抗拒

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