CCL18对人鼻咽癌细胞株增殖、迁移和化疗耐药的影响

发布时间：2018-01-29 23:13

本文关键词： CCL18 鼻咽癌迁移化疗耐药　出处：《重庆医科大学》2013年硕士论文　论文类型：学位论文

【摘要】：目的：研究趋化因子配体18（CCL18）在体外对人鼻咽癌细胞株增殖、迁移能力和化疗耐药性的影响，探讨其临床意义。方法：以人鼻咽癌细胞株HNE-1为研究对象，通过用噻唑蓝(MTT)比色法检测不同浓度的重组CCL18对细胞增殖能力的影响。用Transwell小室体外迁移系统检测CCL18对鼻咽癌细胞的趋化作用。将细胞与CCL18共培育后，加入不同浓度的顺铂，，用MTT比色法检测细胞的半数存活量（LD50），分析CCL18对鼻咽癌细胞化疗耐药的影响。结果：不同浓度的CCL18与鼻咽癌细胞共同培育后，CCL18对鼻咽癌细胞的增殖无明显促进作用（P＞0.05）。在趋化实验中，不同浓度的重组CCL18的各组均能能提高细胞活动能力，促进细胞的迁移（P＜0.05），较对照组有明显促使细胞穿膜的作用(P＜0.05)，并呈现浓度依赖性。经CCL18处理的鼻咽癌细胞对顺铂治疗的敏感性降低，CCL18浓度升高后，达到相同的细胞半数致死量需要更高浓度的顺铂，CCL18提高了细胞对顺铂的化疗耐药性。结论：CCL18可作为鼻咽癌的生物学标志物，其表达与鼻咽癌的侵袭转移和化疗耐药性相关，对鼻咽癌的诊断、治疗和预后判断有一定临床意义。
[Abstract]:Aim: to study the effects of chemokine ligand 18CCL18 on proliferation, migration and chemotherapeutic resistance of human nasopharyngeal carcinoma cell line in vitro. Methods: human nasopharyngeal carcinoma cell line HNE-1 was studied. By using thiazolyl blue (MTT). The effect of different concentrations of recombinant CCL18 on cell proliferation was detected by colorimetric assay. The chemotaxis of CCL18 on nasopharyngeal carcinoma cells was detected by Transwell chamber migration system in vitro. CL18 co-culture. The effect of CCL18 on chemotherapeutic resistance of nasopharyngeal carcinoma cells was analyzed by MTT colorimetric assay. Results: after different concentrations of CCL18 and nasopharyngeal carcinoma cells were co-cultured, CCL18 had no significant effect on the proliferation of nasopharyngeal carcinoma cells (P > 0.05). Different concentrations of recombinant CCL18 could improve cell activity and promote cell migration (P < 0.05), which was more effective than that of control group (P < 0.05). The sensitivity of nasopharyngeal carcinoma cells treated with CCL18 to cisplatin treatment decreased after the concentration of CCL18 increased, and reached the same cell half lethal dose required a higher concentration of cisplatin. CCL18 increases the chemotherapeutic resistance of cells to cisplatin. Conclusion the expression of 1% CCL18 may be a biomarker of nasopharyngeal carcinoma, and its expression is related to invasion, metastasis and chemotherapeutic resistance of nasopharyngeal carcinoma. It has some clinical significance in the diagnosis, treatment and prognosis of nasopharyngeal carcinoma.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R739.63

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