庆大霉素对水平半规管壶腹嵴的高频旋转感受功能损伤及其形态学基础

发布时间：2018-01-30 06:05

本文关键词： 庆大霉素壶腹嵴顶毛细胞频率实验组半规管旋转刺激形态学基础旋转实验高频　出处：《生理学报》2009年06期 　论文类型：期刊论文

【摘要】：本研究旨在探讨庆大霉素对前庭水平半规管壶腹嵴毛细胞的频率选择性感受功能损伤特点及其形态学基础。以豚鼠为研究对象,给予实验组1、2、3连续每天皮下注射庆大霉素(50mg/kg)1周、2周、3周,对照组皮下注射等量生理盐水。停药后用冰水试验和高频旋转实验诱发并记录视频眼震图(videonystagmography,VNG),用低频和高频旋转实验诱发并记录前庭诱发电位(vestibular evoked potential,VsEP)。视频眼震检查记录眼震持续时间和频率,VsEP检查记录P1、P2波潜伏期和波幅(P1、P2分别起源于前庭神经和前庭神经核)。另将实验组豚鼠分批处死,取水平半规管壶腹嵴标本,扫描、透射电镜观察。结果显示:(1)视频眼震检查:冰水刺激后实验组1、2眼震持续时间和频率与对照组差别无统计学意义(P0.05),实验组3未引出明显眼震。高频旋转刺激后,与对照组相比,实验组1眼震持续时间和频率无显著差异(P0.05),而实验组2眼震持续时间缩短和频率下降,差别具有统计学意义(P0.01);实验组3亦未引出明显眼震。(2)VsEP检查:低频旋转后实验组1、2诱发电位参数与对照组无明显差别(P0.05),实验组3未见典型波形。高频旋转刺激后,与对照组相比,实验组1的P1,P2无显著差异(P0.05),而实验组2P1,P2潜伏期延长,两波的波幅均缩短,差异具有统计学意义(P0.01);实验组3未见典型波形。(3)电镜观察:对照组壶腹嵴毛细胞形态未见异常;实验组1壶腹嵴顶毛细胞纤毛略稀疏,毛细胞水肿;实验组2壶腹嵴毛细胞损伤以中央部分坏死为主,外周细胞形态结构基本完整;实验组3毛细胞纤毛完全消失,未见形态完整毛细胞。以上结果提示,庆大霉素主要影响水平半规管壶腹嵴的高频旋转感受功能,对壶腹嵴毛细胞的损伤先以中央顶部为主,逐渐累及嵴顶外周,直至完全损伤壶腹嵴毛细胞,位于前庭感受器壶腹嵴顶部的毛细胞的损伤和丢失可能是高频旋转感受功能损害和缺失的形态学基础。
[Abstract]:The purpose of this study was to investigate the damage characteristics and morphological basis of frequency selective sensory function of gentamicin on vestibular semicircular canal cristae hair cells. (3) Gentamycin 50 mg / kg / day was injected subcutaneously for 1 week and 2 weeks and 3 weeks respectively. The control group was subcutaneously injected with the same amount of normal saline. After withdrawal of the drug, videonystagmography (VNGs) was induced and recorded by ice water test and high frequency rotation test. Vestibular evoked potential were induced and recorded by low frequency and high frequency rotation experiments. Visual nystagmus recording the duration and frequency of nystagmus and the latency and amplitude of P _ (1) P _ 2 wave were recorded by VsEP. P2 originated from vestibular nerve and vestibular nucleus respectively. The guinea pigs in the experimental group were killed in batches. The specimens of horizontal semicircular canal ampullary ridge were taken and scanned. The results showed that the duration and frequency of 1 nystagmus in the experimental group were not significantly different from those in the control group after ice water stimulation (P 0.05). After high frequency rotation stimulation, there was no significant difference in the duration and frequency of nystagmus between the experimental group and the control group (P 0.05). In the experimental group, the duration of nystagmus was shortened and the frequency of nystagmus was decreased, the difference was statistically significant (P 0.01). No obvious nystagmus was induced in experimental group 3. VsEP examination: after low frequency rotation, there was no significant difference in the parameters of evoked potential between experimental group and control group (P 0.05). There was no typical waveform in experimental group 3. After high frequency rotation stimulation, there was no significant difference in P1P 2 between experimental group 1 and control group (P 0.05), but the latency of P 1 P 2 was prolonged in group 2. The amplitudes of the two waves were shortened, and the difference was statistically significant (P 0.01). In experimental group 3, there were no typical waveforms. (3) Electron microscope observation: in control group, there was no abnormal morphology of cristal hair cells in ampulla; In experimental group 1, the ciliated hair cells at the apex of Crista ampulla were slightly sparse and the hair cells were edematous. In experimental group 2, the injury of ampullary cristae was mainly caused by central part necrosis, and the morphology and structure of peripheral cells were basically intact. In experimental group 3, the cilia disappeared completely, but no intact hair cells were found. The above results suggest that gentamicin mainly affects the high-frequency rotatory sensory function of horizontal semicircular canal and ampullary ridge. The damage to the cristae hair cells of ampulla was mainly at the top of the center and gradually involved the periphery of the cristae until the hair cells of the cristae of the ampulla were completely damaged. The damage and loss of hair cells located at the top of the vestibular receptor ampullary ridge may be the morphological basis for the damage and absence of high frequency rotatory sensory function.
【作者单位】：复旦大学附属眼耳鼻喉科医院耳鼻喉科;上海交通大学医学院基础医学院生理学系;上海交通大学医学院医学科学研究院;
【基金】：supported by the National Natural Science Foundation of China(No.30700939) Research Foundation of Shanghai Health Bureau,China(No.2006y09)
【分类号】：R764
【正文快照】： 过去相当长的一段时间里,前庭系统对不同频率旋转的感知特点一直不被重视,近几年,各种不同频段检测方法的出现为研究前庭的频率特性提供了很好的检测手段[1-3]。Sadeghi等[1]利用多种频率的前庭检测手段对单侧迷路切除的动物进行了不同阶段的检测,证实了前庭的频率带宽特性和

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