喉癌与手术切缘组织PCNA和Cdc2表达与局部复发相关性研究

发布时间：2018-01-31 16:47

本文关键词： 早期喉癌手术切缘组织 PCNA Cdc 局部复发回顾性分析　出处：《中华肿瘤防治杂志》2014年21期 　论文类型：期刊论文

【摘要】：目的研究早期喉癌手术切缘组织学检测癌阴性组织PCNA和Cdc2表达与肿瘤局部复发的关系。方法选取2004-01-01-2010-12-31唐山市协和医院85例早期喉癌患者作为研究对象,采用CO2激光手术治疗并进行回顾性研究。采用免疫组织化学方法(SP)对肿瘤及其切缘组织中PCNA和Cdc2的表达进行检测。所有患者均随访2年,对其生存及肿瘤局部复发情况进行观察。结果85例喉癌患者中有14例局部复发(复发组),71例未复发(未复发组)。85例患者肿瘤组织中PCNA阳性表达率为72.94%(62/85),Cdc2阳性表达率为70.59%(60/85)。肿瘤组织PCNA的表达与喉癌的分化程度(χ2=0.001,P=0.979)及分期(χ2=0.132,P=0.716)差异无统计学意义;Cdc2的表达与喉癌的分化程度(χ2=1.803,P=0.179)及分期(χ2=0.232,P=0.630)差异无统计学意义。切缘组织中PCNA和Cdc2阳性率分别为32.94%(28/85)和29.41%(25/85)。局部复发率为16.47%(14/85),喉癌复发组切缘组织中PCNA和Cdc2蛋白表达阳性率分别为64.28%(9/14)和26.76%(19/71);未复发组分别为57.14%(8/14)和23.94%(17/71)。经统计学处理,喉癌复发组织PCNA和Cdc2蛋白阳性表达率差异有统计学意义,χ2=7.454,P0.01;未复发组阴性切缘组织中阳性表达率差异有统计学意义,χ2=6.208,P0.05。结论早期喉癌阴性手术切缘组织中PCNA和Cdc2的表达是预测术后复发的重要生物学标志,癌切缘组织PCNA和Cdc2蛋白联合检测癌阴性组织对判断早期喉癌术后是否发生局部复发具有一定价值。
[Abstract]:Objective to study the relationship between the expression of PCNA and Cdc2 and the local recurrence of laryngeal carcinoma in early laryngeal carcinoma by histological examination of resection margin. Methods from January 2004 to January 2001,2010-12-31, the relationship between the expression of PCNA and Cdc2 and the local recurrence of the tumor was studied. Eighty-five patients with early laryngeal cancer in Yuehe Hospital of Shanshan City were studied. CO2 laser surgery and retrospective study. Immunohistochemical method. The expression of PCNA and Cdc2 in the tumor and its incisor was detected. All the patients were followed up for 2 years. Results there were 14 cases of local recurrence (recurrence group) in 85 cases of laryngeal carcinoma. The positive expression rate of PCNA was 72.94 / 85 in tumor tissues of 85 patients without recurrence (72.94 / 85). The positive expression rate of Cdc2 was 70.59 / 85 / 85.The expression of PCNA and the differentiation of laryngeal carcinoma were observed (蠂 ~ 2 / 0. 001). There was no significant difference between P0.979 and staging (蠂 ~ 2 ~ (2) ~ (0.132)) (P ~ (0.716)). The expression of Cdc2 was correlated with the differentiation of laryngeal carcinoma (蠂 2 / 1.803 / P 0.179) and the stage (蠂 2 / 0.232). The positive rates of PCNA and Cdc2 were 32.94 and 29.41%, respectively. The local recurrence rate was 16.47% 14 / 85). The positive rates of PCNA and Cdc2 protein expression in the resected margin of laryngeal cancer group were 64.28 / 14 and 26.76 / 71 respectively. In the non-recurrence group, 57.14% and 23.94% / 71%, respectively, were statistically analyzed. The positive expression rate of PCNA and Cdc2 protein in recurrent laryngeal carcinoma tissues was significantly different (蠂 ~ 2 ~ (7.454) P ~ (0.01)). There was significant difference in the positive expression rate in the negative margin tissues of the non-recurrence group (蠂 2 / 6.208). Conclusion the expression of PCNA and Cdc2 in early laryngeal carcinoma negative surgical margin is an important biological marker for predicting postoperative recurrence. The combined detection of PCNA and Cdc2 protein in the resection margin of laryngeal carcinoma has some value in judging the local recurrence of early laryngeal carcinoma.
【作者单位】：唐山市协和医院病理科;唐山市人民医院放化科;
【分类号】：R739.65
【正文快照】： 中华肿瘤防治杂志,2014,21(21):1701-1705Chin J Cancer Prev Treat,2014,21(21):1701-1705喉癌是头颈部常见的恶性肿瘤之一,组织学类型以鳞状细胞癌为主。近年来,随着恶性肿瘤发病率的上升,喉癌的发病率也在逐年增加。目前,手术仍是根治早期喉癌的主要方法之一,手术切除是否

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