慢性间歇性缺氧条件下大鼠胰岛功能与mTOR通路相关蛋白表达的变化

发布时间：2018-02-01 07:55

本文关键词： 慢性间歇性低氧(CIH) 胰岛β细胞细胞增殖哺乳动物雷帕霉素靶蛋白　出处：《南京医科大学学报(自然科学版)》2014年07期 　论文类型：期刊论文

【摘要】：目的:探讨慢性间歇性低氧(chronic intermittent hypoxia,CIH)条件下哺乳动物雷帕霉素靶蛋白(mammalian target of rapamycin,mTOR)及相关通路激活对大鼠胰岛β细胞增殖的影响及可能机制。方法:24只成年雄性SD大鼠按随机数字法平均分为两组。CIH组通过低氧舱造模,低氧条件(60 S/N2,60 S/空气,交替充入,保证舱内氧气浓度在5%~21%之间波动,每天8:30~16:30,共35 d)。酶联免疫法检测两组血糖、血清胰岛素水平并计算胰岛素抵抗指数(HOMA-IR)和β细胞胰岛素分泌功能(HOMA-β)。口服葡萄糖法检测两组大鼠糖耐量,免疫荧光法检测胰岛β细胞增殖情况,免疫印迹法检测胰腺组织mTOR及通路相关蛋白指标表达。结果:与对照组相比,CIH组大鼠空腹血糖、血清胰岛素水平及胰岛素抵抗指数升高(P0.01);葡萄糖耐量、胰岛β细胞功能降低(P0.05);ki67染色阳性的β细胞约增加了5倍;胰腺组织mTOR通路相关蛋白激活。结论:CIH条件下大鼠发生胰岛素抵抗、葡萄糖耐量降低、胰岛β细胞功能下降,而mTOR相关通路的激活可能在胰岛β细胞增殖中发挥作用。
[Abstract]:Objective: to study the chronic intermittent hypoxia of chronic intermittent hypoxia. Target of rapamycin. Methods 24 adult male Sprague-Dawley rats were randomly divided into two groups. The hypoxic condition is 60 S / N 2 and 60 S / air, which is filled alternately to ensure that the oxygen concentration in the cabin fluctuates between 5% and 21%, 8: 30% 16: 30% a day. The blood glucose was detected by enzyme-linked immunosorbent assay (Elisa) for 35 days. Serum insulin level and insulin resistance index (HOMA-IRI) and insulin secretion function of 尾 cells were calculated. Glucose tolerance was measured by oral glucose method. Immunofluorescence assay was used to detect the proliferation of islet 尾 cells, and Western blot was used to detect the expression of mTOR and pathway related protein in pancreatic tissue. Results: compared with the control group, the fasting blood glucose of rats in the control group was determined. Serum insulin level and insulin resistance index increased P0.01; Glucose tolerance, 尾 -cell function of islet decreased P0.05; The number of 尾 cells positive for ki67 staining increased by 5 times. Conclusion the insulin resistance, glucose tolerance and 尾 -cell function of pancreatic islets were decreased in rats with mTOR pathway activation. The activation of mTOR related pathway may play a role in the proliferation of islet 尾 cells.
【作者单位】：南京医科大学第一附属医院耳鼻咽喉科;
【基金】：南京医科大学第一附属医院创新团队基金(IRT-16) 教育部留学回国人员科研启动基金(20101174)
【分类号】：R766
【正文快照】： 阻塞性睡眠呼吸暂停综合征(obstructive sleepapnea syndrome,OSAS)在正常成人中发病率为2%~4%[1],以睡眠过程中反复发生上气道阻塞为特点,导致呼吸暂停和睡眠片段化[2],从而引发一系列并发症,如糖尿病、高血压等[3-4]。临床调查研究显示在[Acta Univ Med Nanjing,2014,34(07)

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