RNA干扰沉默Trop2表达对喉癌裸鼠皮下移植瘤的抑制作用

发布时间：2018-02-02 02:20

本文关键词： 喉肿瘤人滋养层细胞表面抗原- RNA干扰裸鼠 Hep-细胞　出处：《临床耳鼻咽喉头颈外科杂志》2014年18期 　论文类型：期刊论文

【摘要】：目的:研究siRNA沉默人滋养层细胞表面抗原-2(Trop2)基因对人喉癌裸鼠皮下移植瘤生长和对Trop2表达的抑制作用。方法:将与Trop2mRNA互补的siRNA通过阳离子脂质体转染Hep-2细胞,以正常Hep-2细胞为对照组,将Hep-2组细胞和Hep-2-siRNA组细胞分别注射于裸鼠皮下,4周后处死裸鼠后测量移植瘤体积并称量移植瘤重量,分别采用免疫组织化学SP法、实时定量PCR和Western blot法检测Trop2mRNA和蛋白的表达。结果:人喉癌裸鼠皮下移植瘤模型建立成功后,Hep-2-siRNA组裸鼠皮下移植瘤的体积增长明显落后于Hep-2对照组,瘤体质量下降(P0.05);Hep-2-siRNA组裸鼠裸鼠皮下移植瘤中Trop2mRNA和蛋白表达较Hep-2组明显下降(P0.05)。结论:siRNA沉默Trop2基因可以下调裸鼠移植瘤中Trop2表达,抑制肿瘤生长。RNA干扰技术可能成为治疗喉癌的一种新途径。
[Abstract]:Objective: to study the silencing of human trophoblast cell surface antigen-2 Trop2 by siRNA. Methods: siRNA complementary to Trop2mRNA was transfected into Hep-2 cells by cationic liposome. Normal Hep-2 cells were used as control group. Hep-2 group and Hep-2-siRNA group were injected subcutaneously into nude mice. After 4 weeks the nude mice were killed and then the volume of transplanted tumor was measured and the weight of transplanted tumor was measured respectively by immunohistochemical SP method. The expression of Trop2mRNA and protein was detected by real-time quantitative PCR and Western blot. Results: the subcutaneous transplanted tumor model of human laryngeal carcinoma was successfully established in nude mice. The volume growth of subcutaneous transplanted tumor in Hep-2-siRNA group was significantly lower than that in Hep-2 control group, and the tumor mass decreased (P 0.05). The expression of Trop2mRNA and protein in subcutaneous transplanted tumor of nude mice in Hep-2-siRNA group was significantly lower than that in Hep-2 group (P0.05). Conclusion silencing Trop2 gene with 1% siRNA can down-regulate the expression of Trop2 in xenografts of nude mice. Inhibition of tumor growth. RNA interference may be a new way to treat laryngeal carcinoma.
【作者单位】：南通大学附属医院耳鼻咽喉科;
【分类号】：R739.65
【正文快照】： 喉鳞状细胞癌(laryngeal squamous cell carci-noma,LSCC)是耳鼻咽喉头颈外科常见的恶性肿瘤,目前喉癌的主要治疗方法是手术治疗,再辅以化疗、放疗等,虽然通过各种治疗方法喉癌的生存率有了明显的提高,但是喉癌的总体生存率还是不高,一直以来人们都在为寻找喉癌的治疗方法而努

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