罗格列酮对人鼻咽癌CNE-2细胞增殖抑制和放射增敏作用的初步研究

发布时间：2018-02-11 06:36

本文关键词： 罗格列酮凋亡鼻咽癌放射增敏细胞周期　出处：《中南大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的：探讨罗格列酮对人鼻咽癌CNE-2细胞增殖抑制和放射增敏作用及可能机制。方法：利用MTT法测定罗格列酮对鼻咽癌CNE-2细胞的半数抑制浓度(IC50)；应用克隆形成法和单靶多击模型拟合放射剂量-细胞存活曲线,观察罗格列酮对鼻咽癌CNE-2细胞株的放射增敏作用；吖啶橙染色与AnnexinV/PI双染流式细胞技术检测细胞凋亡；PI单染流式细胞技术观察罗格列酮对CNE-2细胞周期分布的影响。结果：罗格列酮对在体外培养的CNE-2细胞有不同程度的生长抑制作用,且在一定范围内,随处理时间的延长和给药浓度的增加,其对CNE-2细胞的抑制率逐渐升高。罗格列酮在24h、48h、72h对CNE-2细胞的IC50分别为114.07μmol/L、97.46μmol/L、73.35μmol/L。克隆形成实验显示罗格列酮联合放射线作用于CNE-2细胞表现为剂量曲线左移,SER增加、Do值减低、Dq值变小细胞的放射敏感性增强,罗格列酮对鼻咽癌CNE-2细胞株有明显的放射增敏效应；流式细胞术检测显示罗格列酮在较高浓度可诱导人鼻咽癌CNE-2细胞凋亡在低毒浓度下未能诱导CNE-2细胞凋亡,但能使其阻滞在G2/M期从而导致细胞周期再分布。结论：罗格列酮对CNE-2细胞有增殖抑制和放射增敏作用,放射增敏的机理可能与罗格列酮使CNE-2细胞阻滞在G2/M期并使S期细胞比例减少导致细胞周期再分布有关。
[Abstract]:Aim: to investigate the effect of rosiglitazone on proliferation inhibition and radiosensitization of human nasopharyngeal carcinoma (NPC) CNE-2 cells and its possible mechanism. Methods: the half-inhibitory concentration of rosiglitazone on nasopharyngeal carcinoma (NPC) CNE-2 cells was determined by MTT assay, and the radiation dose-cell survival curve was fitted by clone formation method and single-target multi-shot model. The radiosensitizing effect of rosiglitazone on nasopharyngeal carcinoma (NPC) CNE-2 cell line was observed, and the effect of rosiglitazone on the distribution of CNE-2 cell cycle was observed by acridine orange staining and AnnexinV/PI double staining flow cytometry. Results: rosiglitazone could inhibit the growth of CNE-2 cells cultured in vitro, and in a certain range, with the prolongation of treatment time and the increase of drug concentration, rosiglitazone could inhibit the growth of CNE-2 cells in vitro. The inhibitory rate of rosiglitazone on CNE-2 cells gradually increased. The IC50 of rosiglitazone on CNE-2 cells was 114.07 渭 mol / L, 97.46 渭 mol / L, 73.35 渭 mol / L at 24 h and 48 h / 72 h, respectively. Clonogenic assay showed that rosiglitazone combined with radiation induced by rosiglitazone on CNE-2 cells showed a dose curve of left shift of SER, increase of do value and decrease of DQ. The radiosensitivity of small cells was increased. Rosiglitazone had a significant radiosensitization effect on nasopharyngeal carcinoma CNE-2 cell line, flow cytometry showed that rosiglitazone could induce apoptosis of human nasopharyngeal carcinoma CNE-2 cell line at higher concentration and failed to induce CNE-2 cell apoptosis at low concentration. But it can block it in G 2 / M phase and lead to cell cycle redistribution. Conclusion: rosiglitazone can inhibit the proliferation and radiosensitization of CNE-2 cells. The mechanism of radiosensitization may be related to the cell cycle redistribution caused by rosiglitazone blocking CNE-2 cells in G 2 / M phase and reducing the proportion of S phase cells.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R965;R739.63

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