Eotaxin及CCR3在鼻息肉中的表达及其意义

发布时间：2018-02-15 01:50

本文关键词： Eotaxin CCR3 嗜酸粒细胞鼻息肉免疫组织化学染色　出处：《延边大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的：采用免疫组化染色方法观察在人鼻息肉中嗜酸粒细胞趋化因子(Eotaxin)与嗜酸性粒细胞趋化因子受体(CCehemokine receptor3,CCR3)的表达情况,探讨其临床意义。方法：选择鼻息肉组织28例,鼻中隔矫正术患者的下鼻甲粘膜组织11例作为对照组,采用免疫组织化学染色方法(SABA法)观察Eotaxin与CCR3在鼻息肉与对照组中的表达,并进行统计学分析结果： 1.嗜酸性粒细胞(eosinophieis, EOS)浸润数：HE染色可见鼻息肉组织黏膜层、黏膜下层及血管和腺体周围组织中可见各种炎性细胞浸润,其中以EOS浸润为主(EOS计数：55.64±15.11)。对照组下鼻甲黏膜中可见少量的嗜酸性粒细胞浸润(EOS计数：5.27±3.72)。EOS在鼻息肉组中的浸润数显著高于对照组,差异有统计学意义(P0.01)。 2.免疫组织化学染色片上可见Eotaxin阳性表达位于细胞浆中,呈棕黄色,其分布广,主要表达在黏膜上皮细胞、血管内皮细胞、腺体细胞及各种炎性细胞(Eotaxin阳性细胞数：69.28±19.48)；在对照组中阳性染色弱,分布于黏膜上皮或浸润的炎性细胞中(Eotaxin阳性细胞数：3.82±2.40)。在鼻息肉组中的表达显著多于对照组,且差异有统计学意义(P0.01)。 3.免疫组织化学染色片中细胞膜染成棕黄色的为CCR3阳性细胞,在鼻息肉组织中主要表达在黏膜上皮细胞与黏膜下固有层中浸润的EOS细胞膜上并且染色较深(CCR3阳性表达数：55.79±21.60)。在对照组下鼻甲黏膜中可见少量的CCR3阳性细胞并且染色浅(CCR3阳性表达数：7.45±5.10)。鼻息肉组CCR3的阳性表达数显著多于对照组,且差异有统计学意义(P0.01)。 4.Eotaxin阳性表达数与浸润的EOS数量呈显著正相关(r值为0.883；P值为0.00),具有统计学意义(P0.01)。CCR3阳性表达数和嗜酸粒细胞数呈显著正相关(r值为0.800,P值为0.00),具有统计学意义(P0.01)。Eotaxin与CCR3阳性表达数呈显著正相关(r值为0.776,P值为0.00),具有统计学意义(P0.01)。结论：鼻息肉中可见大量的EOS浸润,Eotaxin和CCR3的表达均明显增强,Eotaxin和CCR3可能参与了EOS的迁移和聚集,并在鼻息肉形成过程中发挥重要作用。
[Abstract]:Objective: to investigate the expression of eotaxin and CCehemokine receptor 3 CCR3 in human nasal polyps by immunohistochemical staining. Methods: 28 cases of nasal polyps and 11 cases of nasal septum correction were selected as control group. The expression of Eotaxin and CCR3 in nasal polyps and control group was observed by immunohistochemical staining. And statistical analysis. Results:. 1. The infiltration of eosinophieis (EOS) in eosinophieis (eosinophieis) was observed in nasal polyp tissue mucosal layer, submucosal layer, blood vessel and periglandular tissue. The number of eosinophils infiltrated in the mucosa of the inferior turbinate in the control group was significantly higher than that in the control group (P 0.01), and the number of eosinophils infiltrated in the nasal polyp group was significantly higher than that in the control group (P < 0.01), and a small amount of eosinophil infiltration was found in the mucosa of the inferior turbinate in the control group. 2. The positive expression of Eotaxin was found in the cytoplasm of the cells, which was brown and widely distributed, mainly in the epithelial cells of the mucous membrane and the endothelial cells of the blood vessel. The number of eotaxin positive cells in glandular cells and various inflammatory cells was 69.28 卤19.48, and the number of eotaxin positive cells in mucosal epithelium or infiltrated inflammatory cells was significantly higher in nasal polyp group than in control group, and the number of eotaxin positive cells in mucosal epithelium or infiltrated inflammatory cells was significantly higher than that in control group. The difference was statistically significant (P 0.01). 3. CCR3 positive cells were stained with brown cell membrane in immunohistochemical staining tablets. In nasal polyps, it was mainly expressed on EOS cell membrane infiltrated in mucosal epithelial cells and submucous lamina propria and stained deeply. The positive expression of CCR3 in nasal polyps was 55.79 卤21.600.A small number of CCR3 positive cells were observed and stained in the mucosa of inferior turbinate in control group. The positive expression of CCR3 in the nasal polyp group was significantly higher than that in the control group, and the positive expression of CCR3 in the nasal polyp group was significantly higher than that in the control group. The difference was statistically significant (P 0.01). 4. There was a significant positive correlation between eotaxin positive expression and the number of infiltrated EOS. The positive correlation between the positive expression of eotaxin and the number of eosinophils was 0.800, P = 0.000.The positive correlation between eotaxin and the number of infiltrated EOS was 0.883 (P = 0.88) (P < 0.05), and the positive correlation between the positive expression of eotaxin and the number of eosinophilic granulocytes was 0.800 (P = 0.004). There was statistical significance between eotaxin and CCR3 positive. There was a significant positive correlation between the number of sexual expression and P value (P = 0.776P = 0.001), which was statistically significant (P 0.01). Conclusion: a large number of EOS infiltrating eotaxin and CCR3 expressions in nasal polyps may be involved in the migration and aggregation of EOS and play an important role in the formation of nasal polyps.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R765.25

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