不同照射模式、C225对CNE-2裸鼠移植瘤抑瘤及Bax、Bcl-2表达研究

发布时间：2018-02-16 14:57

本文关键词： CNE-2 IMRT C225 Bcl-2 Bax　出处：《新疆医科大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的：探讨不同照射模式对人鼻咽低分化鳞癌细胞株(CNE-2)裸小鼠移植瘤抑瘤率的影响,及西妥昔单抗(C225)对CNE-2裸小鼠移植瘤不同照射模式的抑瘤率的影响,以及检测不同照射模式及其联合C225对细胞凋亡相关基因Bax、Bcl-2表达的影响研究。方法：成功构建CNE-2裸小鼠移植瘤模型48只,随机分为空白对照组、急速照射组、模拟IMRT组、C225组、急速+C225组、模拟IMRT+C225组,每组8只；观察各组治疗前后肿瘤抑瘤率的变化情况,并检测细胞凋亡相关基因Bax、Bcl-2的转录水平。C225组给予C2251mg/只,腹腔注射。放疗采用6MV-X线,剂量率300cGy/min。急速照射组：照射剂量20Gy,单次照射,照射完成时间6.67min；模拟IMRT组：照射剂量20Gy,分15次照射,照射完成时间30min。放疗联合C225组在照射结束后给予腹腔内注射C225 1 mg/只。照射后次日及隔天用游标卡尺测量肿瘤基底的最大径,观察时间40天,根据测量结果绘制各实验组的肿瘤生长曲线,计算抑瘤率,并运用RT-PCR法检测细胞凋亡相关基因Bax、Bcl-2的转录水平。结果：(1)肿瘤抑瘤率：C225组与对照组相比抑瘤率无统计学意义；急速照射组、模拟IMRT组、急速+C225组和模拟IMRT+C225组抑瘤率分别为39.32%、27.49%、34.87%、33.76%。模拟IMRT同急速照射组相比有统计学差异(P=0.006).(2)Bax转录水平：空白对照组、急速照射组、模拟IMRT组、C225组、急速+C225组和模拟IMRT+C225组转录水平分别为0.452、0.662、0.558、0.542、0.621、0.555,放疗后Bax表达上调,急速照射组表达高于模拟IMRT组(P=0.005)；使用C225后Bax表达上调(P=0.015)；急速与急速+C225组、模拟IMRT组与模拟IMRT+C225组差异均无统计学意义(P0.005)。(3)Bcl-2转录水平：空白对照组、急速照射组、模拟IMRT组、C225组、急速+C225组和模拟IMRT+C225组转录水平分别为0.629、0.685、0.654、0.625、0.658、0.648,各组差异均无统计学意义(P0.005)。结论：(1)急速照射与模拟IMRT均明显提高了抑瘤率,急速照射作用优于模拟IMRT;单纯C225组无明显抑制肿瘤生长的作用；放疗联合C225较单纯使用放疗对肿瘤生长影响无差异；(2)模拟IMRT照射时间延长,Bax表达较急速照射组相对下调；单纯应用C225后Bax表达上调,提示C225可能具有一定促凋亡作用；放疗联合C225同单独使用放疗相比Bax表达无明显差异；(3)照射及应用C225后Bcl-2表达的相对稳定,不受照射方式及C225的影响；
[Abstract]:Objective: To investigate the different radiation pattern of human nasopharyngeal carcinoma cell line (CNE-2) in nude mice and the inhibition rate of cetuximab (C225) on CNE-2 nude mice with different exposure modes of inhibition rate, and the detection of different radiation pattern and combined with C225 on cell apoptosis study on the effect of Bcl-2 gene Bax expression. Methods: the successful construction of CNE-2 model in nude mice 48, were randomly divided into control group, rapid irradiation group, simulated IMRT group, C225 group, +C225 group, IMRT+C225 group, rapid, simulation, 8 rats in each group; the observation group before and after treatment, tumor suppression rate of tumor changes, and detection of apoptosis related gene Bax, the transcriptional level of.C225 group Bcl-2 C2251mg/, intraperitoneal injection. Radiotherapy using 6MV- X-ray, 300cGy/min. rapid dose rate irradiation group: dose 20Gy, single irradiation, irradiation time 6.67min mode; Quasi IMRT group: dose 20Gy, 15 times of irradiation, irradiation time 30min. radiotherapy combined with C225 group after irradiation given by intraperitoneal injection of C225 1 mg/. After irradiation and the next day the next day with the maximum diameter of the tumor base vernier caliper, observation time of 40 days, according to the measurement results of each experimental group draw tumor growth curve, calculated the inhibition rate and the use of RT-PCR method to detect apoptosis related gene Bax, Bcl-2 expression. Results: (1) the tumor inhibition rate: C225 group compared with the control group, the inhibition rate was not statistically significant; rapid irradiation group, simulated IMRT group, +C225 group and IMRT+C225 group the rapid simulation of tumor suppression rate were 39.32%, 27.49%, 34.87%, 33.76%. simulation of IMRT has statistical difference compared with rapid irradiation group (P=0.006). The transcription level of Bax (2): blank control group, rapid irradiation group, simulated IMRT group, C225 group, +C225 group and IMRT+C225 group the rapid simulation of turn Record levels were 0.452,0.662,0.558,0.542,0.621,0.555, increased expression of Bax after radiotherapy, rapid irradiation group was higher than that in group IMRT (P=0.005) simulation; the upregulation of the expression of Bax after using C225 (P=0.015); rapid and rapid +C225 group, IMRT group and IMRT+C225 group simulation simulation showed no significant difference (P0.005). (3) the transcription level of Bcl-2: blank the rapid control group, irradiation group, simulated IMRT group, C225 group, +C225 group and IMRT+C225 group the rapid simulation of the transcription level of 0.629,0.685,0.654,0.625,0.658,0.648 respectively, each group showed no significant difference (P0.005). Conclusion: (1) the rapid irradiation and Simulation of IMRT obviously improves the inhibition rate, rapid irradiation effect is better than pure C225 IMRT simulation; no significant inhibition of tumor growth; radiotherapy combined with C225 compared with the use of radiotherapy on tumor growth and influence no difference; (2) simulation of IMRT irradiation time prolonged, the expression of Bax was rapidly The irradiation group relative reduction; expression of Bax with C225, suggesting that C225 may play a pro apoptotic effect; C225 radiotherapy combined with radiotherapy alone, compared with no significant differences of Bax expression; (3) the expression of Bcl-2 and C225 after irradiation application is relatively stable, not affected by irradiation and C225;

【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R739.6

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