内源性大麻素系统在慢性间歇低氧大鼠心肌肥厚病理过程中的作用

发布时间：2018-02-24 11:12

本文关键词： 慢性间歇低氧钙/钙调素依赖性蛋白激酶II 心肌营养素- 心肌肥厚内源性大麻素受体利莫那班　出处：《中国循环杂志》2014年07期 　论文类型：期刊论文

【摘要】：目的:探讨内源性大麻素系统在慢性间歇低氧大鼠心肌肥厚中作用及使用内源性大麻素受体拮抗剂利莫那班后对其的影响。方法:48只雄性Wistar大鼠,根据实验设计在实验4、6周时随机分组,即正常对照4周组、6周组,低氧4周组、6周组,低氧+利莫那班即拮抗低氧4周组、6周组,共6组,随机取8只大鼠,处死后测左心室质量指数(LVMI),光学显微镜下观察心肌细胞形态变化,免疫组化测钙/钙调素依赖性蛋白激酶II(CaMKII)、心肌营养素-1(CT-1)表达。结果:低氧4周组、6周组分别与正常对照4周组、6周组相比,LVMI、光镜下心肌细胞肥厚及免疫组化下CaMKII、CT-1均明显升高,组间比较差异均有统计学意义(P0.001);与正常对照组、低氧组比较,低氧拮抗组上述各项明显高于正常对照组但低于低氧组,各组间比较差异均有统计学意义(P0.001)。结论:慢性间歇低氧通过导致内源性大麻素系统紊乱诱发心肌肥厚,使用其拮抗剂利莫那班干预后可有效减轻上述病理过程的发生。
[Abstract]:Objective: to investigate the effect of endogenous cannabinoid system on myocardial hypertrophy in chronic intermittent hypoxic rats and the effect of rimonaban, an endogenous cannabinoid receptor antagonist, on it. According to the experimental design, the rats were randomly divided into 6 groups at the 4th week of experiment, that is, normal control group (4 weeks), hypoxia group (6 weeks), hypoxia group (4 weeks group), hypoxia group (6 weeks group), and hypoxic rimonaban group (4 weeks and 6 weeks group), a total of 6 groups were randomly selected, and 8 rats were randomly selected. Left ventricular mass index (LVMI) was measured after death, and the morphologic changes of myocardial cells were observed under optical microscope. The expression of calcium / calmodulin-dependent protein kinase III-CaMKII-1 and myocardial nutrient-1CT-1 were measured by immunohistochemistry. Results: compared with the control group, LVMIs, myocardial hypertrophy and CaMKIICT-1 were significantly increased under light microscope in the 4-week hypoxia group and the 6-week control group respectively, and the expression of CaMKIICT-1 was significantly increased under light microscope, compared with that in the control group at 4 weeks and 6 weeks after hypoxia, respectively, and the expression of CaMKIICT-1 was significantly increased under light microscope. Compared with normal control group and hypoxic group, the above items in hypoxia antagonistic group were significantly higher than those in normal control group but lower than that in hypoxia group. Conclusion: chronic intermittent hypoxia can induce myocardial hypertrophy by causing endogenous cannabinoid system disorder, and the use of rimonaban, an antagonist, can effectively reduce the occurrence of the above pathological process.
【作者单位】：山西医科大学第二医院呼吸内科;
【基金】：山西省归国留学基金委员会项目(2013-116)
【分类号】：R766

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