白介素-18在大鼠视网膜新生血管形成中作用的实验研究

发布时间：2018-02-26 20:14

本文关键词： 白介素-18 视网膜新生血管血管内皮生长因子　出处：《石河子大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的：探讨白介素-18对大鼠视网膜中血管内皮生长因子(VEGF)及新生血管形成的影响。方法：采用同日出生7天的SD大鼠幼鼠50只,共100只眼,随机选取40只统一编号后置于750±20mL/L氧仓中连续饲养5d,建立高氧诱导的血管增生性视网膜病变模型。剩余10只为正常对照组,不给予任何处理,正常环境中饲养。建模后幼鼠随机分为试验低浓度组(10只)：给予双眼玻璃体内注射1ul的IL-18(10ng/u1)。实验高浓度组(10只)：给予双眼玻璃体内注射1ul的IL-18(50ng/u1)。生理盐水组(10只)：给予双眼玻璃体内注射lul的生理盐水。高氧对照组(10只)：双眼不给予任何处理。于第21d将五组小鼠颈椎脱臼处死,取新鲜视网膜组织进行HE染色组织学切片上观察并计数突破视网膜内界膜进入玻璃体的新生血管内皮细胞核数目、免疫组织化学法检测VEGF的表达,CD34标记血管内皮细胞观察视网膜血管改变,比较各组间差异。结果：①HE染色显示,在组织切片上可见正常对照组(1.40±0.70)内界膜清晰、连续,极少见新生血管内皮细胞核。而其他组均有较多新生血管内皮细胞核突破视网膜内界膜进入玻璃体腔内,但实验组明显低于生理盐水组(14.9±1.12)及高氧对照组(14.7±2.26),并且在用高浓度实验组(6.50±1.27)少于低浓度实验组(10.1±1.52)。②正常对照组(0.30±0.48)极少见VEGF阳性表达,实验组、生理盐水组及高氧对照组均发现较多表达VEGF的阳性细胞,在生理盐水组(3.70±0.48)及高氧对照组(3.50±0.71)表达最明显,而实验组VEGF阳性表达明显低于生理盐水组及高氧对照组(P0.05),且低浓度实验组(2.60±0.52)与高浓度实验组(1.90±0.74)间比较差异有统计学意义(P0.05)。⑧正常对照组(1.3±0.48)极少见增生的毛细血管,实验组、生理盐水组及高氧对照组发现较多CD34标记的增生的毛细血管,在生理盐水组(6.9±0.11)及高氧对照组(6.6±0.97)增生最明显,实验组相对较少,且低浓度实验组(3.8±0.79)与高浓度实验组(2.8±0.79)间之间比较差异有统计学意义(P0.05)。结论：IL-18玻璃体腔内注射可抑制血管内皮细胞的增殖及迁移,从而抑制视网膜新生血管的形成。
[Abstract]:Aim: to investigate the effects of interleukin-18 (IL-18) on vascular endothelial growth factor (VEGF) and neovascularization in rat retina. Methods: a total of 50 SD rats (100 eyes) were born on the same day. 40 rats were randomly selected and fed in 750 卤20ml / L oxygen chamber for 5 days to establish hyperoxia-induced angioproliferative retinopathy model. The remaining 10 rats were normal control group without any treatment. After modeling, young rats were randomly divided into low concentration group (n = 10): intravitreous injection of 1ul / L IL-18ng / u ~ (-1), experimental high concentration group (n = 10): intravitreous injection of 1ul / L ~ (50 ng / u ~ (-1)). Normal saline group (n = 10) were given intravitreous injection of 1ul (IL-18g / u ~ (-1)) and normal saline (n = 10). 10 rats in hyperoxia control group were given intravitreous injection of normal saline with lul. The rats in the hyperoxia control group were killed by cervical dislocations on the 21st day without any treatment in both eyes. The number of neovascularized endothelial nuclei entering vitreous body through the membrane of retinal inner boundary was observed and counted on the histological sections of fresh retina tissue by HE staining. Immunohistochemical method was used to detect the expression of VEGF and CD34 labeled vascular endothelial cells (VECs) to observe the changes of retinal blood vessels and to compare the differences among the groups. Results the results showed that the normal control group (1.40 卤0.70) had a clear and continuous internal boundary membrane. The nucleus of neovascularization endothelial cells was rarely seen in the other groups, while in other groups, the nucleus of neovascularization entered the vitreous cavity through the inner limiting membrane of the retina. However, the positive expression of VEGF in the experimental group was significantly lower than that in the saline group (14.9 卤1.12) and the hyperoxia control group (14.7 卤2.26), and the expression of VEGF in the high concentration group (6.50 卤1.27) was less than that in the low concentration group (10.1 卤1.52n.2) in the normal control group (0.30 卤0.48). Positive cells expressing VEGF were found in saline group and hyperoxia control group. The expression of VEGF was most obvious in saline group (3.70 卤0.48) and hyperoxia control group (3.50 卤0.71). The positive expression of VEGF in the experimental group was significantly lower than that in the saline group and hyperoxia control group (P 0.05), and there was a significant difference between the low concentration group (2.60 卤0.52) and the high concentration group (1.90 卤0.74). In the saline group and hyperoxia control group, more CD34 labeled hyperplastic capillaries were found, especially in the saline group (6.9 卤0.11) and hyperoxia control group (6.6 卤0.97). There was significant difference between the low concentration group (3.8 卤0.79) and the high concentration group (2.8 卤0.79). Conclusion Intravitreous injection of% IL-18 can inhibit the proliferation and migration of vascular endothelial cells and thus retina neovascularization.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R774.1

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