鼻咽癌高危人群血浆EBV-DNA定量分析

发布时间：2018-02-28 19:26

  本文关键词： 鼻咽癌 高危人群 EBV-DNA 鼻咽癌筛查　出处：《中华肿瘤防治杂志》2014年17期 　论文类型：期刊论文

【摘要】：目的:探讨鼻咽癌高危人群E病毒DNA(Epstein-Barr virus DNA,EBV-DNA)的存在情况及其在鼻咽癌早期诊断中的应用价值。方法:2009-08-13-2010-07-27在广东省中山市小榄镇开展鼻咽癌筛查,ELISA法检测16 712名EBV抗体,确定鼻咽癌高危人群386名,同时收集鼻咽癌低危人群273名。收集同期中山市人民医院初诊鼻咽癌患者62例。荧光定量PCR方法检测高危人群血浆EBV-DNA,并随访1年,比较分析高危人群EBV-DNA定量检测的应用价值。结果:初筛鼻咽癌高危人群EBV-DNA阳性率为12.2%(47/386),高于鼻咽癌低危人群的3.3%(9/273),而低于初诊鼻咽癌患者的91.9%(57/62),3组差异有统计学意义,P0.001。随访复查时,血清学持续高危人群EBV-DNA阳性率为9.7%(18/186),而血清学转变为非高危人群的EBV-DNA阳性率为3.1%(3/97),差异有统计学意义,P=0.045。筛查人群中血清学诊断鼻咽癌的阳性预测值为5.6%(32/572),而在鼻咽癌高危人群中增加EBV-DNA检测,其阳性预测值提高到44.6%(29/65)。结论:鼻咽癌高危人群血浆EBV-DNA定量检测能对血清学EBV抗体检测进行有效的补充,可大大提高筛查准确性,对鼻咽癌高危人群的监测具有重要作用。
[Abstract]:Objective: to investigate the presence of E virus DNA(Epstein-Barr virus DNA (EBV-DNA) in high risk population of nasopharyngeal carcinoma (NPC) and its value in early diagnosis of nasopharyngeal carcinoma. Methods: 16 712 EBV antibodies were detected by Elisa in Xiaolan Town, Zhongshan City, Guangdong Province. 386 cases of high risk population of nasopharyngeal carcinoma and 273 cases of low risk population of nasopharyngeal carcinoma were collected. In the same period, 62 cases of nasopharyngeal carcinoma were first diagnosed in Zhongshan people's Hospital. The plasma EBV-DNA was detected by fluorescence quantitative PCR and followed up for 1 year. Results: the positive rate of EBV-DNA in high risk population of nasopharyngeal carcinoma was 12.2%, which was significantly higher than that of low risk group (3.33% / 273), but lower than that of group 91.9% / 62% in nasopharyngeal carcinoma (NPC). There was a significant difference (P0.001) in the follow-up, and the positive rate of EBV-DNA in the high risk population of nasopharyngeal carcinoma (NPC) was 12.2% (P < 0.05), which was significantly higher than that in the low risk group (P < 0.01). The positive rate of EBV-DNA was 9. 7 / 18 / 1862 in the population with continuous high risk of serology, while the positive rate of EBV-DNA in the serological transition to non high risk group was 3. 1 / 97, the difference was statistically significant (P 0. 045). The positive predictive value of serological diagnosis of nasopharyngeal carcinoma in the screening population was 5. 6 / 572 and was high in nasopharyngeal carcinoma (NPC). Increased EBV-DNA detection in people at risk, Conclusion: the quantitative detection of plasma EBV-DNA in high risk population of nasopharyngeal carcinoma can effectively supplement the detection of serological EBV antibody, improve the accuracy of screening, and play an important role in the monitoring of high risk population of nasopharyngeal carcinoma.
【作者单位】： 中山大学附属中山医院肿瘤研究所;中山大学肿瘤防治中心·华南肿瘤学国家重点实验室实验研究部;
【基金】：“十一五”国家科技支撑计划(2006BAI02A11) 中山市科技计划(20083A183)
【分类号】：R739.63
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本文编号：1548629