大鼠视神经钳夹伤模型的建立及磁共振成像运用初探

发布时间：2018-03-01 11:34

本文关键词： 大鼠视神经夹持伤模型磁共振成像病理过程　出处：《眼科新进展》2014年03期 　论文类型：期刊论文

【摘要】：目的通过多时间点活体磁共振成像及组织取材病理检查,探讨钳夹法建立大鼠视神经损伤模型的可靠性及核磁共振成像在视神经损伤模型中的应用价值。方法健康SD大鼠36只,随机分为对照组(6只)和实验组(30只)。使用夹持力50g夹持钳持续钳夹10 s的方法建立大鼠单侧视神经损伤模型,在损伤后3 d、5 d、7 d、14 d、2个月分别对受损侧视神经进行磁共振成像扫描,并随后取视网膜及视神经进行病理检测。结果磁共振成像检查结果显示,对照组大鼠双侧视神经粗细均匀,周围无明显病变信号。损伤后3 d,实验组损伤侧可出现视神经肿胀、增粗,伴随眼球后方软组织水肿,并在随后5～14 d内呈进行性加重;至损伤后2个月,可观察到损伤侧视神经球后段萎缩。HE染色检查结果显示,实验组损伤后3～7 d,视神经出现大量空泡变性,神经纤维稀疏,胶质细胞增生明显,视网膜神经纤维层水肿明显,神经节细胞层出现核固缩和空泡;损伤后14 d,梗死灶内胶质细胞增生,纤维组织相对增多,神经纤维层结构仍显疏松;损伤后2个月,可见神经纤维增生,胶质细胞数目明显减少。对照组大鼠视神经纤维排列密集、规则,视网膜层次清晰,神经纤维层厚度均匀。结论固定压力钳夹法能建立稳定的大鼠视神经损伤模型,可获取随时间变化的视神经及视网膜细胞病理改变图像。同时,磁共振成像能显示创伤后视神经水肿及后期萎缩的变化过程,具有一定的研究价值和临床应用潜力。
[Abstract]:Objective to explore the reliability of the rat optic nerve injury model established by clamp method and the application value of magnetic resonance imaging (MRI) in optic nerve injury model. Methods 36 healthy SD rats were included in this study. The rats were randomly divided into control group (n = 6) and experimental group (n = 30). The unilateral optic nerve injury model was established by using 50 g clamp for 10 s. The injured optic nerve was scanned by magnetic resonance imaging (MRI) at 3 days, 5 days, 7 days and 14 days after injury, and then the retina and optic nerve were taken out for pathological examination. Results the results of magnetic resonance imaging showed that the thickness of bilateral optic nerve in the control group was uniform. 3 days after injury, the optic nerve swelling and thickening appeared in the experimental group, accompanied by edema of the soft tissue behind the eyeball, and gradually aggravated within 514 days after the injury, until 2 months after the injury. It was observed that the optic nerve in the experimental group showed a large number of vacuolar degeneration, sparse nerve fibers, obvious proliferation of glial cells and edema of retinal nerve fiber layer after 3 days of injury. At 14 days after injury, glial cells proliferated, the fibrous tissue increased relatively, the structure of nerve fiber layer was still loose, and the proliferation of nerve fibers was observed at 2 months after injury. The number of glial cells decreased obviously. In the control group, the optic nerve fibers were arranged densely, the retinal layer was clear and the thickness of the nerve fiber layer was uniform. Conclusion the fixed pressure clamp method can establish a stable model of optic nerve injury in rats. At the same time, Mr imaging can show the process of optic nerve edema and late atrophy after trauma, which has certain research value and clinical application potential.
【作者单位】：昆明医科大学第二附属医院中心实验室;
【基金】：国家自然科学基金资助(编号:31160206)~~
【分类号】：R779.1

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