白细胞介素-17和基质金属蛋白酶-9在中耳胆脂瘤的表达及意义

发布时间：2018-03-02 20:29

本文选题：IL-17　切入点：MMP-9　出处：《安徽医科大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的探讨白细胞介素17(interleukin17IL-17)、基质金属蛋白酶9(matrix metalloproteinase9MMP-9)在中耳胆脂瘤的表达及意义,分析它们在胆脂瘤上皮骨质破坏中的作用,为临床治疗提供新的治疗方向。方法收集蚌埠医学院第一附属医院耳鼻咽喉科收治胆脂瘤型中耳炎病人术中胆脂瘤标本35例和作为对照组的10例非胆脂瘤型中耳炎患者的外耳道皮肤标本,制成石蜡切片,同时观察病人的骨质破坏程度。采用免疫组化法检测白细胞介素17、基质金属蛋白酶9在35例胆脂瘤上皮中的表达和在10例外耳道皮肤中的表达情况。结果IL-17阳性表达定位于细胞质内,35例胆脂瘤组织标本组织中均有表达,呈不同程度的高、中、弱表达。外耳道皮肤标本中几乎没有表达,两者间有显著性差异(P0.001)。MMP-9阳性细胞主要定位于细胞浆,在胆脂瘤上皮各层细胞均有表达,外耳道皮肤组织为弱阳性表达,两者间有显著性差异(P0.001)。中耳胆脂瘤组织中IL-17与MMP-9的表达存在正相关关系(r=0.437,P0.01)。结论IL-17、MMP-9在胆脂瘤上皮的高表达,与胆脂瘤的侵袭能力有显著相关性.提示IL-17、MMP-9高表达是胆脂瘤引起骨质破坏吸收的主要因素之一。IL-17、 MMP-9在中耳胆脂瘤疾病的发生及骨质破坏的形成过程中起到的重要作用。那么通过以IL-17、MMP-9为靶点,为此提供理论依据,做相关治疗,对减缓疾病的发展和并发症的发生、改善预后并提高患者的生活质量将会有一定的临床意义。
[Abstract]:Objective to investigate the expression and significance of interleukin 17 interleukin 17 (IL 17) and matrix metalloproteinase 9 (MMP-9) in middle ear cholesteatoma, and to analyze their roles in the destruction of cholesteatoma epithelium and provide a new therapeutic direction for clinical treatment. Methods 35 cases of cholesteatoma type otitis media treated in Department of Otolaryngology, first affiliated Hospital of Bengbu Medical College and 10 cases of non-cholesteatoma type otitis media were collected and made into paraffin sections. The expression of interleukin-17 (IL-17) and matrix metalloproteinase-9 (MMP-9) in the epithelium of 35 cases of cholesteatoma and in the skin of 10 cases of external auditory canal were detected by immunohistochemistry. Results the positive expression of IL-17 was found in 35 cases of cholesteatoma tissue, which was high, medium and weak in different degree, but almost no expression in the skin of external auditory canal. There was significant difference between the two groups. The positive cells were mainly located in cytoplasm and expressed in all layers of cholesteatoma epithelium, but weakly positive in the skin of external auditory canal. The expression of IL-17 and MMP-9 in middle ear cholesteatoma was positively correlated with the expression of MMP-9. Conclusion IL-17 MMP 9 is highly expressed in the epithelium of cholesteatoma. It is suggested that the high expression of IL-17 and MMP-9 is one of the main factors of bone destruction and absorption caused by cholesteatoma. IL-17 and MMP-9 play an important role in the development of middle ear cholesteatoma and the formation of bone destruction. Then by targeting IL-17, MMP-9, Therefore, providing theoretical basis and relevant treatment will be of clinical significance in slowing down the development of diseases and complications, improving the prognosis and improving the quality of life of patients.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R764

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