骨髓间充质干细胞对视网膜缺血再灌注损伤中Fas和FasL表达的影响

发布时间：2018-03-03 00:34

本文选题：视网膜缺血再灌注损伤　切入点：骨髓间充质干细胞移植　出处：《眼科新进展》2014年04期 　论文类型：期刊论文

【摘要】：目的探讨骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSC)移植对大鼠视网膜缺血再灌注损伤(retinal ischemia reperfusion injury,RIRI)凋亡相关因子Fas和FasL表达的影响。方法体外贴壁筛选法培养大鼠BMSC。将39只健康成年SD大鼠随机分成正常组3只、模型组18只、BMSC移植组18只。各组大鼠均选择右眼作为实验眼建立大鼠RIRI模型,正常组不做任何处理。另2组大鼠于RIRI后1 h分别给予玻璃体腔注射PBS缓冲液(模型组)、BMSC(BMSC移植组),并于RIRI后2 h、6 h、12 h、24 h、48 h、72 h各随机处死3只SD大鼠,迅速摘取右眼。免疫组织化学法检测大鼠视网膜组织Fas和FasL的阳性细胞数情况。结果正常视网膜组织中Fas极少表达,FasL视网膜全层低表达。RIRI后2 h、6 h、12 h、24 h、48 h、72 h,模型组Fas阳性细胞数分别为(20.5±0.9)个·mm-2、(263.6±15.6)个·mm-2、(583.5±15.8)个·mm-2、(1184.6±45.5)个·mm-2、(684.5±3.5)个·mm-2、(80.6±4.5)个·mm-2,BMSC移植组分别为(19.8±0.8)个·mm-2、(238.5±14.7)个·mm-2、(473.6±13.6)个·mm-2、(964.5±23.7)个·mm-2、(632.6±5.8)个·mm-2、(72.5±6.3)个·mm-2;模型组FasL阳性细胞数分别为(42.2±0.9)个·mm-2、(57.3±0.9)个·mm-2、(365.8±7.5)个·mm-2、(1004.3±17.9)个·mm-2、(793.5±8.6)个·mm-2、(210.6±4.9)个·mm-2,BMSC移植组分别为(40.5±0.8)个·mm-2、(55.6±0.7)个·mm-2、(351.2±6.8)个·mm-2、(992.8±6.0)个·mm-2、(683.4±12.9)个·mm-2、(206.3±4.7)个·mm-2。与模型组比较,BMSC移植组各时间点Fas、FasL的阳性细胞数明显减少。Fas在6~48 h各组差异有统计学意义(P0.05),FasL在12~48 h各组差异有统计学意义(P0.05)。结论 RIRI后Fas/FasL表达水平升高,而玻璃体腔内注射BMSC抑制Fas/FasL的表达,证明BMSC移植对RIRI有治疗作用。
[Abstract]:Objective to investigate the effect of bone marrow mesenchymal stem cells (BMSCs) transplantation on the expression of Fas and FasL, the apoptosis-related factors of retinal ischemia reperfusion injury in rats with retinal ischemia-reperfusion injury. Methods BMSCs were cultured by adherent screening in vitro. Adult SD rats were randomly divided into normal group (n = 3) and control group (n = 3). There were 18 rats in the model group and 18 rats in the BMSC transplantation group. All the rats in each group were selected as experimental eyes to establish RIRI model. The other two groups were given intravitreous injection of PBS buffer solution 1 h after RIRI (model group), and 3 SD rats were randomly killed at 2 h, 6 h, 12 h, 24 h, 48 h and 72 h after RIRI, respectively. The number of Fas and FasL positive cells in rat retina was detected by immunohistochemical method. Results the low expression of Fas in the whole retinal layer of normal retina was rarely detected. 2 h after 2 h, 12 h, 12 h, 24 h and 48 h and 72 h after RIRI, the model was established. The number of Fas positive cells in the transplantation group was 263.6 卤15.6 卤263.6 卤15.6) 路m ~ (-2) 路m ~ (-2), respectively, 1184.6 卤45. 5 卤45. 5 卤45. 5) 路m ~ (-2) and 684.5 卤3. 5 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) of Fas positive cells in the transplantation group were 19.8 卤0. 8 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 卤7 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-2) 路m ~ (-1)??? The number of FasL positive cells in the BMSC transplantation group was significantly lower than that in the BMSC transplantation group at 648 h after 648 h. The number of FasL positive cells was 55.6 卤0.7. The number of FasL was 351.2 卤6.8. the number of FasL was significantly lower in BMSC transplantation group than that in BMSC transplantation group (P 0.05) at 1248 h. There was a significant difference in the number of Fas-FasL positive cells between the two groups at 1248 h (P 0.05) and P 0.05 (P 0.05) at 1248 h. The number of Fas-FasL positive cells was significantly lower in BMSC transplantation group than that in the model group at each time point (P < 0.05). The number of FasL positive cells in BMSC transplantation group was significantly lower than that in BMSC transplantation group at 648 h. There were significant differences in the number of Fas-FasL positive cells between two groups (P 0.05) at 1248 h. Conclusion the expression of Fas/FasL increased after RIRI. Intravitreal injection of BMSC inhibits the expression of Fas/FasL, indicating that BMSC transplantation has therapeutic effect on RIRI.
【作者单位】：潍坊医学院眼科教研室;潍坊医学院附属医院眼科中心;山东省鲁南眼科医院;
【基金】：山东省自然科学基金资助(编号:Y2007C084) 山东省教育厅基金资助(编号:J07YE02)~~
【分类号】：R774.1

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