轴突导向因子-1mRNA在糖尿病大鼠视网膜中的表达

发布时间：2018-03-04 16:33

  本文选题：Netrin-1　切入点：糖尿病视网膜病变　出处：《中南大学》2011年硕士论文　论文类型：学位论文

【摘要】：目的检测链脲佐菌素(Streptozotocin, STZ)诱导的糖尿病大鼠模型视网膜中Netrin-1mRNA的表达变化,探讨Netrin-1在糖尿病视网膜病变(Diabetic retinopathy, DR)中可能存在的发病机理。 方法将20只成年雄性Sprague-Dawley (SD)大鼠随机分为正常对照组(Normal control, NC)与糖尿病组(Diabetic model, DM),每组各10只,用提前预冷的PH4.5的柠檬酸缓冲液溶解STZ, DM组按照60mg/kg体重剂量一次性腹腔注射新鲜配制的1%STZ,对照组腹腔注射0.5mL柠檬酸缓冲液。注药48h后测空腹尾静脉血糖≥16.67mmol/L者定义为糖尿病组大鼠,每个月测量糖尿病大鼠体重及血糖。糖尿病大鼠成模后3个月处死,采用Trizol法提取大鼠视网膜组织总RNA,应用逆转录聚合酶链反应(RT-PCR)技术检测NC组和DM组大鼠视网膜中Netrin-1mRNA的表达差异。 结果(1)STZ诱导的大鼠糖尿病模型的建立：STZ诱导糖尿病大鼠成模率为90%,1只大鼠注射STZ后血糖未升高而淘汰,剩余9只糖尿病组大鼠注射STZ后出现糖尿病多饮、多食、多尿、体重下降的典型“三多一少”糖尿病症状,随时间延长毛发逐渐失去光泽,行动较迟缓,精神稍差。与正常对照组相比,糖尿病组大鼠的血糖值明显升高,两者之间有显著性差异(p0.01)；体重增长较正常对照组明显下降,两者之间有显著性差异(p0.01)；3个月时糖尿病大鼠均发生了明显的白内障。(2)RT-PCR检测结果均显示,Netrin-1mRNA在正常对照组大鼠视网膜中弱表达,在糖尿病组中表达较对照组明显增强,两者比较有统计学意义(p0.05)。 结论STZ诱导糖尿病大鼠是一种简便易行的制作糖尿病视网膜病变的动物模型；Netrin-1mRNA在糖尿病诱导3个月的大鼠视网膜中表达较正常对照组明显增强,提示Netrin-1参与了早期糖尿病视网膜病变的发生发展,可能与视网膜血管的通透性增加有关。
[Abstract]:Objective to detect the expression of Netrin-1mRNA in the retina of diabetic rats induced by streptozotocin (STZ) and to explore the possible pathogenesis of Netrin-1 in diabetic retinopathy. Methods Twenty adult male Sprague-Dawley rats were randomly divided into normal control group (NC) and diabetic model (DM) with 10 rats in each group. STZs were dissolved with citric acid buffer of precooled PH4.5 in advance. In DM group, fresh 1 STZ was injected intraperitoneally according to 60 mg / kg body weight, and 0.5 mL citric acid buffer was injected intraperitoneally in control group. 48 hours after injection, fasting caudal vein blood glucose 鈮，

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