血红素加氧酶-1在糖尿病视网膜病变患者外周血单个核细胞中的表达

发布时间：2018-03-08 16:29

本文选题：糖尿病视网膜病变　切入点：血红素加氧酶-　出处：《眼科新进展》2014年01期 　论文类型：期刊论文

【摘要】：目的观察血红素加氧酶-1(heme oxygenase-1,HO-1)在糖尿病视网膜病变(dia betic retinopathy,DR)患者外周血单个核细胞(peripheral blood mono-nuclear cell,PBMC)中的表达。方法选择糖尿病病程大于6个月的2型糖尿病患者共64例,根据临床表现和诊断将其分为无糖尿病视网膜病变(non-diabetic retinopathy,NDR)组18例及DR组46例其中DR组中包括非增生型DR(non-proliferative diabetic retinopathy,NPDR)患者24例及增生型DR(proliferative retinopathy,PDR)患者22例。选择同期行体检的健康志愿者20人作为对照。采用实时荧光定量聚合酶链式反应(quantitative real-time polymerase chain reac tion,qRT-PCR)检测各组PBMC中HO-1 mRNA的表达水平,比较各组之间HO-1 mRNA表达水平的差异,并分析HO-1 mRNA表达水平与各项理化指标之间的相关性。结果 qRT PCR检测发现,DR组、NDR组以及对照组PBMC中HO-1 mRNA相对表达量分别是0.015±0.007、0.021±0.010、0.027±0.013,差异有统计学意义(F=10.888,P0.001)。其中,DR组HO-1 mRNA的相对表达量明显低于NDR组(P=0.014);NDR组与对照组比较差异无统计学意义(P=0.125);PDR组患者PBMC中HO-1 mRNA相对表达量为0.012±0.007,显著低于NPDR组(0.018±0.007)(t=-3.052,P=0.004)。ln(HO-1)mRNA表达水平与收缩压(β=-0.011,P=0.001)及糖化血红蛋白(β=0.088,P=0.008)之间存在显著相关性。结论 HO-1 mRNA在DR患者及PDR患者PBMC中的表达显著降低,提示HO-1可能作为一个保护性因素参与DR的发生及病情进展。
[Abstract]:Objective to investigate the expression of heme oxygenase-1 (HO-1) in peripheral blood mononuclear cells (PBMC) of patients with diabetic retinopathy (betic). According to the clinical manifestation and diagnosis, it was divided into 18 cases of non-diabetic retinopathy group and 46 cases of Dr group, including 24 cases of non-proliferative DR(non-proliferative diabetic retinopathy and 22 cases of proliferative DR(proliferative retinopathy. Quantitative real-time polymerase chain reac qRT-PCRs were used to detect the expression of HO-1 mRNA in PBMC of 20 healthy volunteers. To compare the expression level of HO-1 mRNA between groups, Results the relative expression of HO-1 mRNA in PBMC of Dr group and control group was 0.015 卤0.007 卤0.021 卤0.010 卤0.027 卤0.013 respectively, the difference was statistically significant (P 0.001). The relative expression of HO-1 mRNA in PBMC of NDR group was 0.012 卤0.007, which was significantly lower than that of NDR group (0.018 卤0.007), and was significantly lower than that of NPDR group (0.018 卤0.007 ~ 3.052P 0.004. LnHO-1 mRNA) and systolic pressure (尾 -0.011 P0. 001) and glycosylated hemoglobin (尾 -0.08P0. 008). Conclusion the expression of HO-1 mRNA in PBMC of Dr patients and PDR patients was significantly decreased. The results suggest that HO-1 may be a protective factor in the pathogenesis and progression of Dr.
【作者单位】：南方医科大学;广东省眼病防治研究所广东省人民医院眼科;广东省医学科学院;广东省人民医院医学研究部;
【基金】：国家自然科学基金资助(编号:81371031) 广东省医学科研基金资助(编号:B2011013) 广州市珠江科技新星专项基金资助(编号:2011J2200050)~~
【分类号】：R587.1;R774.1

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