成年小鼠嗅觉功能与嗅上皮中成熟嗅觉神经元数量的实验研究

发布时间：2018-03-10 01:36

本文选题：嗅觉障碍　切入点：嗅觉标记蛋白　出处：《中国临床解剖学杂志》2014年02期 　论文类型：期刊论文

【摘要】：目的探讨成年BALB/C小鼠维持嗅觉功能所需嗅上皮中成熟嗅神经元的数量,研究小鼠嗅上皮中嗅神经元数量与小鼠嗅觉功能的相关性。方法用0.7%的Triton X-100灌注8~10周大小的BALB/C小鼠鼻腔以诱导小鼠嗅觉障碍,分别于灌鼻后第3,7,21,49,56天行觅食实验检测小鼠觅食行为的改变,并联合免疫荧光染色(IFC)的方法,检测小鼠嗅上皮(OE)中成熟嗅神经元(ORNs)的数量与其嗅觉功能的相关性。结果小鼠经Triton X-100灌注鼻腔后第3、7天,其觅食时间明显延长(F=32.04,P0.001),Bonferroni法两两比较示第3,7天觅食时间均长于对照组及造模后第21、49、56天组,P0.001差异有统计学意义。嗅上皮中成熟嗅神经元(ORNs)的数量亦于第3、7天为低(F=223.97,P0.001),觅食时间与嗅上皮成熟ORNs的数量呈负相关(r=-0.757,P0.001)。小鼠嗅觉障碍于造模后第21天左右开始恢复,此时嗅上皮中OMP(+)细胞占28.66%。结论小鼠嗅觉功能与其嗅上皮中成熟ORNs数量具有明显的相关性;当成年BALB/C小鼠嗅上皮中成熟ORNs数量恢复至对照组的28.66%时即可使小鼠嗅觉功能得以恢复。
[Abstract]:Objective to investigate the number of mature olfactory neurons in olfactory epithelium of adult BALB/C mice for maintaining olfactory function. To study the correlation between the number of olfactory neurons in the olfactory epithelium of mice and the olfactory function of mice. Methods 0.7% Triton X-100 was used to infuse the nasal cavity of 8-week-old BALB/C mice to induce olfactory disorders in mice. The foraging behavior of mice was detected by feeding experiment on the 3rd day after nasal administration, and the method of immunofluorescence staining (IFC) was used to detect the changes of foraging behavior in mice. The correlation between the number of mature olfactory neurons (ORNs) and the olfactory function of olfactory epithelium in mice was measured. Results the mice were perfused with Triton X-100 in nasal cavity 3 days after 7 days. The feeding time was significantly prolonged by Bonferroni method. The results showed that the foraging time on the 3rd day was longer than that in the control group and the control group on the 21th day after modeling. The number of the mature olfactory neurons in the olfactory epithelium was also lower than that in the 37th day (P 0.001), and the number of the mature olfactory neurons in the olfactory epithelium was also lower than that in the control group and the control group on the 21st day (P 0.001), and the number of the mature olfactory neurons in the olfactory epithelium was also lower than that in the control group (P 0.001). There was a negative correlation between the feeding time and the number of mature ORNs in olfactory epithelium. At this time, OMP () cells in olfactory epithelium accounted for 28.66. Conclusion the olfactory function of mice has a significant correlation with the number of mature ORNs in olfactory epithelium. When the number of mature ORNs in olfactory epithelium of BALB/C mice returned to 28.66% of the control group, the olfactory function of mice could be restored.
【作者单位】：佛山市第一人民医院耳鼻咽喉-头颈外科;中山大学附属第一医院耳鼻咽喉科医院;
【基金】：广东省科技计划基金(2009B030801202)
【分类号】：R765.6

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