鼠眼碱烧伤后羊膜移植对角膜缘干细胞增殖效应的研究

发布时间：2018-03-11 07:16

  本文选题：碱烧伤　切入点：羊膜移植　出处：《泸州医学院》2010年硕士论文　论文类型：学位论文

【摘要】： 目的：制备SD大鼠眼碱烧伤动物模型,行羊膜移植术,观察P63、增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)在角膜缘处的表达,进一步了解羊膜对角膜缘干细胞(LSCs)增殖效应的影响。方法：健康SD大鼠40只40眼,实验组标本20只,20眼,行眼碱烧伤加羊膜移植术；对照组标本20只,20眼,单纯行眼碱烧伤；术后第1、2、3、4周分别取角膜缘组织进行固定、石蜡包埋、切片,采用免疫组化技术检测角膜缘组织中P63、PCNA的表达情况。结果：羊膜移植组角膜缘组织中P63、PCNA表达情况显著高于对照组,羊膜移植组和对照组相比较,有统计学意义(P0.05)；羊膜移植术后1周、2周、3周和4周P63、PCNA蛋白在角膜缘干细胞的表达不一致。呈线性趋势,1周达到高峰,以后逐渐降低。 结论：眼碱烧伤后行羊膜移植术,对角膜缘干细胞有明显的保护作用和并可促进干细胞的增殖表达。
[Abstract]:Objective: to establish an animal model of ocular alkali burn in SD rats, and to observe the expression of P63, proliferating cell nuclear antigen-PCNA in the limbus of cornea after amniotic membrane transplantation. Methods: 40 eyes of healthy SD rats and 20 eyes of experimental group were treated with ocular alkali burn and amniotic membrane transplantation, and 20 eyes of control group were treated with ocular alkali burn and amniotic membrane transplantation. The corneal limbal tissue was fixed, paraffin embedded and sectioned at the 1st week and 2nd week after operation. The expression of PCNA in the limbal tissue was detected by immunohistochemical technique. Results: the expression of PCNA in the limbal tissue of amniotic membrane transplantation group was significantly higher than that in the control group, and the expression of PCNA in the limbal tissue of the amniotic membrane transplantation group was significantly higher than that in the control group. Compared with the control group, the amniotic membrane transplantation group had statistical significance (P 0.05), and the expression of P63 PCNA protein in limbal stem cells was not consistent at 2 weeks, 3 weeks and 4 weeks after amniotic membrane transplantation. The expression of PCNA protein in limbal stem cells reached the peak in a linear trend at 1 week, and decreased gradually after the first week of amniotic membrane transplantation. Conclusion: amniotic membrane transplantation after ocular alkali burn can protect limbal stem cells and promote the proliferation and expression of stem cells.
【学位授予单位】：泸州医学院
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R779.6

【参考文献】

相关期刊论文 前10条

1 Mehdi Hosseini Tehrani;Asoo Ali Mahmoudi;Hassan Hashemi;Syed Jafar Oskouee;Javad Amuzadeh;Mohammad Taher Rajabi;Masoomeh Taherzadeh;Hamideh Shenazandi;;活体异体结膜缘和羊膜移植治疗化学性眼外伤造成的角膜缘干细胞缺失(英文)[J];国际眼科杂志;2008年06期

2 邓宏伟;姚晓明;齐卫;;表皮生长因子与纤维蛋白封闭剂复合物对碱烧伤后眼表修复的实验研究[J];国际眼科杂志;2009年04期

3 邹美英;黄艳燕;;羊膜移植治疗早期眼表碱性化学伤的临床观察[J];国际眼科杂志;2009年07期

4 胡庆军,周力,车筑平;增殖细胞核抗原在胃癌中的表达及其临床意义[J];贵州医药;2001年02期

5 于蓝;;自体角膜缘干细胞移植治疗翼状胬肉[J];局解手术学杂志;2006年02期

6 李红;苏敏;黄悦;胡蓉;;PCNA在新西兰大耳兔角膜及角膜缘的表达[J];局解手术学杂志;2009年05期

7 易敬林;钟文贤;;不同来源人角膜缘干细胞的体外培养研究[J];江西医学院学报;2005年06期

8 杨得雄;;36例重度角膜碱烧伤的治疗效果分析[J];临床眼科杂志;2005年04期

9 白靓;周余来;成岩;顾国贞;;兔角膜缘干细胞的研究进展[J];现代生物医学进展;2007年10期

10 武英;王丽聪;;维生素C不同给药方式对早期兔角膜碱烧伤的疗效观察[J];山西医药杂志(下半月刊);2008年04期

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