Akt抑制剂对氧诱导视网膜新生血管形成的作用

发布时间：2018-03-11 08:03

本文选题：Akt抑制剂　切入点：视网膜新生血管　出处：《重庆医科大学学报》2015年04期 　论文类型：期刊论文

【摘要】：目的:观察Akt抑制剂对低氧环境下视网膜新生血管形成的抑制作用,探讨Akt活性的抑制对于新生血管抑制的作用。方法:将鼠龄为7 d的C56BL/6J小鼠14只置于浓度为(75±2)%高氧舱环境中生活5 d,然后返回正常氧环境中。14只小鼠于出氧舱后当日被随机平均分为实验组和对照组各7只,实验组小鼠玻璃体腔内注射1.5μl Akt抑制剂,对照组小鼠玻璃体腔内注射1.5μl PBS。在P17,实验组和对照组小鼠FITC-Dextran左心室造影视网膜铺片了解视网膜的血管形态的改变以及小鼠组织学切片观察突破视网膜内界膜的血管内皮细胞核数量。结果:视网膜铺片结果显示实验组与对照组相比较,视网膜可见新生血管芽,但是新生血管丛数量及面积明显减少,荧光渗漏明显减轻,但无灌注区无明显的减小;组织学切片结果表明与对照组相比较,Akt抑制剂注射实验组突破视网膜内界膜的血管内皮细胞核数量明显减少,实验组突破视网膜内界膜的血管内皮细胞核数量为(14±3.0)个,对照组为(55±3.0)个,差异具有统计学意义(P=0.001)。结论:在缺氧环境下运用Akt抑制剂能够抑制视网膜新生血管的发生,但Akt抑制剂不减少无灌注区的面积。
[Abstract]:Aim: to observe the inhibitory effect of Akt inhibitor on retinal neovascularization in hypoxic environment. To investigate the effect of inhibition of Akt activity on neovascularization. Methods: fourteen C56BL / 6J mice, aged 7 days, were exposed to 75 卤2% hyperoxia chamber for 5 days, then returned to normal oxygen environment. 14 mice were exposed to oxygen chamber. They were randomly divided into experimental group (n = 7) and control group (n = 7). In the experimental group, 1.5 渭 l Akt inhibitor was injected into the vitreous cavity of the mice. In the control group, 1.5 渭 l PBSwas injected into the vitreous cavity of the mice. In the P17, the experimental group and the control group were given FITC-Dextran left ventricular angiography to understand the changes of retinal vascular morphology and to observe the blood breakthrough of the inner retinal membrane by histological section of the mice. Results: the results of retina preparation showed that the experimental group was better than the control group. Retinal neovascularization bud was seen, but the number and area of neovascularization plexus were obviously decreased, fluorescence leakage was reduced obviously, but no significant decrease was found in no perfusion area. The results of histological section showed that compared with the control group, compared with the control group, the number of vascular endothelial nuclei in the experimental group with Akt inhibitor injection was significantly reduced, and the number of vascular endothelial nuclei in the experimental group that broke through the retinal inner boundary membrane was 14 卤3.0. The difference was statistically significant in the control group (55 卤3.0). Conclusion: Akt inhibitor can inhibit retinal neovascularization in hypoxic environment, but Akt inhibitor does not decrease the area of no perfusion area.
【作者单位】：重庆医科大学附属第一医院眼科;中南大学湘雅医院眼科;
【分类号】：R774.1

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