内淋巴积水对豚鼠外周前庭频率感受功能的损伤研究

发布时间：2018-03-13 12:42

  本文选题：豚鼠　切入点：动物模型　出处：《复旦大学》2011年硕士论文　论文类型：学位论文

【摘要】：第一部分豚鼠内淋巴积水模型的建立 目的：探讨豚鼠内淋巴积水模型的制作方法,为后续实验奠定基础。方法：健康豚鼠30只,随机分为对照组、实验组1、实验组2,各10只,实验组1、实验组2行右侧内淋巴囊切除及部分内淋巴囊阻塞以建立内淋巴积水动物模型,对照组右侧施行假手术,于术后四周、术后八周分别处死实验组1、实验组2,术后八周处死对照组,取双侧听泡进行切片并染色。结果：对照组及左侧耳蜗均未出现膜迷路积水,实验组1、实验组2出现程度不同膜迷路积水,前庭膜向前庭阶膨出。实验组2较实验组1显示前庭膜膨出更严重。结论：内淋巴囊切除及部分内淋巴囊阻塞术可以导致豚鼠内淋巴积水,有效建立了膜迷路积水的动物模型。 第二部分豚鼠眼震电图的记录 目的：探讨豚鼠的眼震电图的记录方法,从而了解内淋巴积水对豚鼠外周前庭频率感受功能的损伤特点。方法：健康豚鼠30只,随机分为正常对照组、前庭破坏组、实验组,各10只,实验组行右侧内淋巴囊切除及部分内淋巴囊阻塞以建立内淋巴积水动物模型,前庭破坏组行前庭破坏术。用冰水试验和旋转试验诱发并记录正常对照组、前庭破坏组的眼震电图,于术后四周用冰水试验和旋转试验诱发并记录实验组的眼震电图。眼震电图记录眼震持续时间和频率。结果：冰水试验正常对照组可引出明显眼震,并持续较长时间。实验组术后四周冰水刺激未引出眼震,前庭破坏组冰水试验无眼震。在旋转试验中,前庭破坏组未引出明显眼震。正常对照组和实验组术后四周时左、右眼震慢相速度无统计学差异(P0.05)。在甩头试验中,前庭破坏组无明显眼动,正常对照组和实验组术后四周时左、右眼动速度无统计学差异(P0.05)。结论：内淋巴积水主要影响前庭器官的低频旋转感受功能,高频、高速旋转无明显影响。 第三部分内淋巴积水豚鼠各时期眼震变化的记录 目的：探讨内淋巴豚鼠不同时期眼震的变化情况,从而了解内淋巴积水不同时期对前庭功能的损伤特点。方法：健康豚鼠40只,随机分为对照组、术后两周组、术后四周组、术后八周组4组,术后组均行右侧内淋巴囊切除及部分内淋巴囊阻塞以建立内淋巴积水动物模型,于相应时间段用冰水试验和旋转试验诱发并记录眼震电图。并同期处死豚鼠,取水平半规管、上半规管、后半规管、椭圆 囊、球囊标本,进行扫描、透射电镜观察。结果：冰水试验时术后两周组有4只豚鼠(40%)引出眼震,但是眼震较微弱,持续时间较对照组短、眼震速度较慢。其余术后各组均未引出明显眼震。旋转试验时对照组、术后两周组、术后四周组、术后八周组左、右眼震慢相速度无明显差异(P0.05)。甩头试验时,对照组、术后两周组、术后四周组、术后八周组左、右眼动速度无明显差异(P0.05)。电镜中可见术后两周时毛细胞无明显改变,四周时部分区域毛细胞排列不规整,纤毛变短、倒伏,Ⅱ型毛细胞肿胀、空泡,Ⅰ型毛细胞形态较完整。八周时部分区域出现毛细胞的缺失,Ⅱ型毛细胞出现变形、皱缩,Ⅰ型毛细胞形态无明显变形。结论：内淋巴积水主要影响前庭器官的低频旋转感受功能,对前庭毛细胞的损伤多以Ⅱ型毛细胞为主。Ⅱ型毛细胞的损伤和缺失可能是低频旋转感受功能损害和缺失的主要细胞学基础。
[Abstract]:The first part of the model of endolymphatic hydrops in guinea pigs
Objective: To explore the method of making guinea pig endolymphatic hydrops model, lay the foundation for the subsequent experiments. Methods: 30 guinea pigs were randomly divided into control group, experimental group 1, experimental group 2, 10 rats in each experimental group 1, experimental group 2 underwent right hepatectomy and partial endolymphatic sac endolymphatic sac occlusion to establish endolymphatic hydrops animal model, the control group underwent sham operation on the right side, four weeks after surgery, eight weeks after operation respectively. The experimental group 1, experimental group 2, control group were sacrificed eight weeks after operation, the bilateral auditory vesicle were sliced and stained. Results: the control group and the left cochlea were not hydrolabyrinth, experiment group 1, experimental group 2 different degrees of hydrolabyrinth, vestibular membrane forward bulging. The court order 2 in experimental group than in the experimental group 1 showed more severe vestibular meningocele. Conclusion: endolymphatic sac excision and endolymphatic sac occlusion can lead to guinea pig endolymphatic hydrops, established a membranous labyrinth An animal model of water accumulation.
The record of second parts of guinea pig nystagmus
Objective: To explore the method of recording electronystagmogram of guinea pigs, so as to understand the injury characteristics of endolymphatic hydrops in guinea pig vestibular function of frequency. Methods: 30 guinea pigs were randomly divided into normal control group, vestibular damage group, experimental group, 10 rats in each experimental group underwent right hepatectomy and partial in endolymphatic sac in order to establish the obstruction of endolymphatic sac endolymphatic hydrops animal model of vestibular damage group underwent vestibular destruction. With ice water test and rotation test induced and recorded in normal control group, damage group vestibular nystagmus, at four weeks after surgery with ice water test and rotation test induced and electronystagmography recording experimental group electronystagmogram duration were recorded. And the frequency of ice water test. Results: the normal control group can lead to obvious nystagmus, and lasting a long time. After the experimental group around the ice water stimulation did not elicit vestibular nystagmus, nystagmus. No damage group ice test In the rotarod test, vestibular damage were not elicited obvious nystagmus. Normal control group and experimental group four weeks after surgery when left, there was no significant difference in right nystagmus slow phase velocity (P0.05). In the head test, no obvious damage to vestibular eye group, normal control group and experimental group after four weeks left, no significant difference right eye velocity (P0.05). Conclusion: endolymphatic hydrops mainly affects the vestibular organ low-frequency rotation sensation function, high frequency, high rotating speed has no obvious effect.
Changes of nystagmus in third parts of endolymphatic hydrops in guinea pigs
Objective: To investigate the changes of guinea pig endolymphatic nystagmus, so as to understand the endolymphatic hydrops damage characteristics of vestibular function in different period. Methods: 40 guinea pigs were randomly divided into control group, two weeks after surgery group, postoperative 4 weeks group, eight weeks after the operation of 4 groups, postoperative groups the right part of the endolymphatic sac excision and endolymphatic sac occlusion to establish endolymphatic hydrops animal model, in the corresponding time period with ice water test and rotation test induced and recording electronystagmogram and over the same period. The guinea pigs were sacrificed, the horizontal semicircular canal, anterior semicircular canal, posterior semicircular canal, oval
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