用阻塞性睡眠呼吸暂停低通气综合征动物模型研究vaspin与胰岛素信号通路的相关性

发布时间：2018-03-14 07:32

本文选题：阻塞性睡眠呼吸暂停低通气综合征　切入点：Vaspin　出处：《中国病理生理杂志》2014年11期 　论文类型：期刊论文

【摘要】：目的:通过建立阻塞性睡眠呼吸暂停低通气综合征(OSAHS)大鼠模型,检测其vaspin的血清学以及mRNA、蛋白水平的表达特点,阐述脂肪因子vaspin在OSAHS发生过程中的作用机制。方法:用慢性间歇低氧(CIH)模拟OSAHS。健康雄性Wistar大鼠22只,随即分为空白对照(control)组和CIH组。每天将CIH组大鼠放入间歇性低氧舱内,共8周。实验前后应用大鼠尾压测量仪测量血压。实验结束后处死动物,检测空腹血糖(FPG)、总胆固醇(TC)、甘油三酯(TG)、空腹胰岛素(FINS)、vaspin和脂联素。应用实时荧光定量PCR和蛋白印迹法(Western blotting)检测vaspin mRNA以及vaspin、Akt、p-Akt蛋白水平。结果:(1)慢性间歇性缺氧下血气结果:缺氧最低点时大鼠动脉血氧分压23.4 mm Hg~32.8 mm Hg,最低血氧饱和度范围为40.8%~65.9%,恢复舱内氧浓度至21%后,测得动脉血氧分压为74.5 mm Hg~102.9 mm Hg,动脉血氧饱和度为93.8%~98.9%。(2)实验前后2组大鼠之间比较体重均无显著差异;血压变化实验前2组大鼠尾动脉收缩压无显著差异,实验后2组大鼠之间比较,CIH组大鼠收缩压明显高于正常对照组。CIH组和control组比较,FPG、FINS、TG和TC均升高,差别有统计学意义(P0.05)。(3)Pearson相关分析显示,血清vaspin水平与FPG、FINS、HOMA-IR和TC呈正相关。(4)CIH组大鼠脂肪组织的vaspin mRNA和蛋白质的表达水平均增加,差别有统计学意义(P0.01);Akt mRNA表达水平无明显改变,但CIH组的p-Akt蛋白水平较control组降低。结论:OSAHS动物模型中vaspin;水平的改变可能在胰岛素抵抗发生过程中具有一定作用。
[Abstract]:Objective: through the establishment of obstructive sleep apnea hypopnea syndrome (OSAHS) rat model, detection of vaspin serological and mRNA, expression of the protein level, this fat factor vaspin in OSAHS mechanism in the process. Methods: the chronic intermittent hypoxia (CIH) simulated OSAHS. 22 healthy male Wistar rats were randomly divided into control (control) group and CIH group. The rats in the CIH group every day intermittent hypoxia cabin, a total of 8 weeks. Before and after the experiment using the rat tail pressure measuring blood pressure measuring instrument. The experimental animals were killed after animal testing, fasting blood glucose (FPG), total cholesterol (TC), triglyceride (TG), fasting insulin (FINS), vaspin and adiponectin. Using real-time fluorescence quantitative PCR and Western blotting were used to detect vaspin mRNA (Western blotting) and vaspin, Akt, p-Akt protein level. Results: (1) blood gas results in chronic intermittent hypoxia under hypoxia: the lowest point When the arterial oxygen pressure of 23.4 mm Hg~32.8 mm Hg, the lowest oxygen saturation range is 40.8%~65.9%, recovery of oxygen concentration to 21% in the cabin, the artery blood oxygen partial pressure of 74.5 mm Hg~102.9 mm Hg 93.8%~98.9%., arterial oxygen saturation (2) between the 2 groups before and after the experimental rats compared with body weight had no significant difference the change of blood pressure in 2 groups before experiment; rat tail artery systolic blood pressure had no significant difference between the 2 groups of rats after the experiment, the rats of group CIH systolic pressure was significantly higher than the normal control group,.CIH group and control group FPG, FINS, TG and TC were increased, the difference was statistically significant (P0.05 (). 3) Pearson correlation analysis showed that the serum level of vaspin and FPG, FINS, HOMA-IR and TC were positively correlated. (4) the expression level of vaspin mRNA and protein in adipose tissue of rats in CIH group were increased, the difference was statistically significant (P0.01); Akt mRNA expression level was not changed, but the CIH group p-Akt The protein level is lower than that in the control group. Conclusion: the changes in the level of vaspin in the OSAHS animal model may play a role in the process of insulin resistance.

【作者单位】：山东大学第二医院内分泌科;
【基金】：济南市高校自主创新计划(No.201202034)
【分类号】：R766

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