超声波提高大鼠视网膜血管消化铺片的效率

发布时间：2018-03-15 01:37

本文选题：糖尿病视网膜病变　切入点：大鼠　出处：《眼科新进展》2014年03期 　论文类型：期刊论文

【摘要】：目的将超声波应用于大鼠视网膜血管消化铺片技术,提高糖尿病视网膜病变的形态学研究质量。方法取SD大鼠25只,尾静脉注射55 mg·kg-1链脲佐菌素制作I型大鼠糖尿病模型,随机均分2组,胰蛋白酶机械振荡消化组(每隔20 min使用摇床振荡2 min)和超声波(16.5 kHz)辅助胰蛋白酶振荡消化组,分别制作视网膜血管消化铺片,PAS常规染色,比较铺片成功率和光镜观察铺片形态学。结果 8周后,将血糖值稳定于16.7 mmol·L-1以上的20只SD大鼠纳入研究,超声波辅助胰蛋白酶振荡消化法比胰蛋白酶机械振荡消化法的铺片成功率高(分别为85%和55%,P0.05)。在理想的全视网膜血管铺片上可发现典型的形态学变化,如周细胞核固缩和无细胞毛细血管。结论超声波辅助胰蛋白酶制作视网膜血管消化铺片成功率高且简单有效。
[Abstract]:Objective to improve the morphologic study of diabetic retinopathy in rats by using ultrasonic wave to improve the quality of diabetic retinopathy. Methods 25 SD rats were injected with 55 mg 路kg-1 streptozotocin via tail vein to make type I diabetic model. Two groups were randomly divided into two groups: the trypsin mechanical oscillating digestion group (using shaking bed for 2 minutes every 20 min) and the ultrasonic assisted trypsin oscillating digestion group (16.5 kHz). The retinal vascular digesting tablets were prepared by pas routine staining, respectively. Results after 8 weeks, 20 SD rats whose blood glucose values were above 16.7 mmol 路L ~ (-1) were included in the study. Ultrasonic assisted trypsin oscillatory digestion was more successful than trypsin mechanical oscillatory digestion (85% and 55, respectively). Typical morphological changes could be found on the ideal whole retinal vascular patch. Conclusion Ultrasound assisted trypsin has a high success rate and is simple and effective in the preparation of retinal vascular digestibility.
【作者单位】：沈阳何氏眼科医院;天津医科大学基础医学院超微病理室;天津医科大学眼科研究所;
【基金】：天津医科大学校级青年基金(编号:2013KYQ09)~~
【分类号】：R587.2;R774.1

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