顺铂导致螺旋神经节细胞凋亡中pp38MAPK和pERK信号蛋白的作用

发布时间：2018-03-19 14:27

本文选题：螺旋神经节细胞　切入点：顺铂　出处：《听力学及言语疾病杂志》2014年04期 　论文类型：期刊论文

【摘要】：目的初步探讨丝裂素活化蛋白激酶(mitogen-activated proteins kinase,MAPK)信号转导通路中磷酸化p38MAPK蛋白(pp38MAPK)和磷酸化ERK蛋白(pERK)在顺铂耳毒性中的作用。方法选取20只健康豚鼠随机分为两组,每组10只,实验组腹腔注射顺铂4mg·kg-1·d-1,连续4天,对照组腹腔注射等量生理盐水。最后一次给药后24小时内处死动物、取材,HE染色后光镜下观察内耳螺旋神经节细胞形态学变化;透射电镜下观察螺旋神经节细胞的凋亡情况;免疫组化染色法观察螺旋神经节细胞pp38MAPK和pERK蛋白的表达。结果实验组豚鼠螺旋神经节细胞数目减少、体积减小,排列散乱,出现凋亡改变,而对照组螺旋神经节细胞无明显凋亡改变。pp38MAPK主要分布在螺旋神经节细胞胞浆,对照组表达较弱(平均光密度值为0.12±0.04),实验组表达增强(平均光密度值为0.24±0.07),两组差异有统计学意义(t=4.581,P0.05)。pERK在螺旋神经节细胞的胞浆有表达,对照组的平均光密度值为0.27±0.08,实验组的平均光密度值为0.25±0.08,两组差异无统计学意义(t=-0.673,P0.05)。结论 pp38MAPK可能参与了顺铂导致豚鼠螺旋神经节细胞的凋亡。
[Abstract]:Objective to investigate the mitogen activated protein kinase (mitogen-activated proteins, kinase, MAPK) phosphorylation of p38MAPK protein signal transduction pathway (pp38MAPK) and phosphorylated ERK protein (pERK) on the ototoxicity of cisplatin in rats. Methods 20 healthy guinea pigs were randomly divided into two groups, 10 rats in each group, the experimental group by intraperitoneal injection of cisplatin 4mg kg-1 D-1, for 4 consecutive days, the control group were injected with saline. The last administration within 24 hours after the death of animal specimens, HE staining was used to observe the changes of the inner ear spiral ganglion cell morphology under light microscope; to observe the apoptosis of spiral ganglion cells under transmission electron microscope; the expression of immunohistochemical staining of pp38MAPK and pERK protein in spiral ganglion cells in the experimental group. The decrease in the number of spiral ganglion cells in guinea pig, reduce the volume, disorder, apoptosis, while the control group had no obvious spiral ganglion cells Apoptosis of.Pp38MAPK was mainly distributed in spiral ganglion cells, weak expression in the control group (the average optical density value of 0.12 + 0.04), experimental group (enhanced expression of the average optical density value of 0.24 + 0.07), there was significant difference between two groups (t=4.581, P0.05).PERK expression in the cytoplasm of spiral ganglion cells. The average optical density value of the control group is 0.27 + 0.08, the average optical density value of the experimental group was 0.25 + 0.08, there was no significant difference between the two groups (t=-0.673, P0.05). Conclusion pp38MAPK may be involved in cisplatin induced apoptosis of spiral ganglion cells in guinea pigs.

【作者单位】：南京医科大学附属南京医院;南京市第一医院耳鼻咽喉科;中国协和医科大学中国医学科学院北京协和医院耳鼻咽喉科;
【基金】：南京市医学科技发展课题(YKK12093)资助
【分类号】：R764

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