翼状胬肉中血管内皮生长因子、内皮抑素的表达以及新生血管生成情况

发布时间：2018-03-21 11:58

本文选题：翼状胬肉　切入点：血管平衡　出处：《安徽医科大学学报》2014年05期 　论文类型：期刊论文

【摘要】：目的通过研究翼状胬肉组织中新生血管数量、促进血管生成因子以及抑制血管生长因子的表达情况,探讨翼状胬肉组织中促进血管形成和抑制血管生成的情况。方法手术获得正常的结膜组织35例(对照组)及翼状胬肉组织40例(实验组),用免疫组化及Western blot的方法分别检测两组样本中新生血管密度(MVD)、血管内皮生长因子(VEGF)、内皮抑素(Endostatin)的表达情况。其中,CD31是标记组织MVD的指标。结果①免疫组化法结果显示:在翼状胬肉组织中MVD的含量明显高于正常结膜组织;VEGF在正常球结膜组织中仅有少量阳性表达,在翼状胬肉组织中阳性细胞明显增多,着色显著加深;翼状胬肉组织中上皮细胞基底细胞层中的Endostatin抗原阳性表达则明显少于正常球结膜组织。②Western blot结果显示人正常球结膜与翼状胬肉中VEGF及Endostatin蛋白表达存在差异。翼状胬肉组织中VEGF蛋白/β-actin(1.46±0.59)显著高于正常球结膜组/β-actin(0.46±0.45),差异有统计学意义(P0.05);翼状胬肉组织中Endostatin蛋白/β-actin(0.58±0.11)显著低于正常球结膜组/β-actin(1.54±0.12),差异有统计学意义(P0.05)。结论在翼状胬肉中发现其MVD及促血管生长因子的表达高于正常结膜组织,而血管抑制因子的表达含量降低,这一血管平衡的打破可能是翼状胬肉的发病基础之一。
[Abstract]:Objective to study the expression of angiogenic factors and angiogenesis factors in pterygium. Methods 35 cases of normal conjunctival tissue (control group) and 40 cases of pterygium tissue (experimental group) were obtained by immunohistochemistry and Western blot. Methods the expression of neovascularization density (MVD), vascular endothelial growth factor (VEGF) and endostatin (endostatin) in the two groups were detected respectively. The expression of MVD in normal conjunctiva was significantly higher than that in normal conjunctiva. In pterygium, the positive cells increased significantly and the staining deepened significantly. The positive expression of Endostatin antigen in the basal cell layer of epithelial cells in pterygium was significantly lower than that in the normal bulbar conjunctiva tissue. 2 Western blot results showed that there was a difference in the expression of VEGF and Endostatin protein between human normal bulbar conjunctiva and pterygium and pterygium pterygium. VEGF protein / 尾 -actinine in pterygium was significantly higher than that in conjunctiva conjunctiva / 尾 -actinine 0.46 卤0.45, and Endostatin protein / 尾 -actinin 0.58 卤0.11 in pterygium was significantly lower than that in normal conjunctiva / 尾 -actinine 1.54 卤0.120.Conclusion it is found in pterygium that the protein / 尾 -actinin in pterygium is significantly lower than that in normal conjunctiva / 尾 -actinin (1.54 卤0.12). Conclusion there is a significant difference in pterygium between pterygium and pterygium. The expression of MVD and vascular growth factor was higher than that of normal conjunctiva. The decrease of the expression of angiostatin may be one of the pathogenesis of pterygium.
【作者单位】：安徽医科大学研究生学院;安徽医科大学第二附属医院眼科;
【基金】：安徽省自然科学基金项目(编号:1208085MH178) 安徽省高校自然科学基金重点项目(编号:KJ2013A147) 安徽医科大学校基金(编号:2013xkj024)
【分类号】：R777.33

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